Potentiometric flow injection sensing system for determination of heparin based on current-controlled release of protamine

被引：14

作者：

Lei, Jiahong ^{[1
,2
]}

Ding, Jiawang ^{[1
,2
]}

Chen, Yan ^{[1
]}

Qin, Wei ^{[1
]}

机构：

[1] Chinese Acad Sci, Shandong Prov Key Lab Coastal Environm Proc, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2015年 / 858卷

基金：

中国国家自然科学基金;

关键词：

Potentiometry; Polycation-sensitive membrane electrodes; Protamine; Heparin; Current-controlled release; Flow injection analysis; SENSITIVE MEMBRANE-ELECTRODE; MOLECULAR-WEIGHT HEPARIN; ELECTROCHEMICAL SENSOR; RESPONSE MECHANISM; POLYION SENSORS; LABEL-FREE; POLYANIONS; BLOOD;

D O I：

10.1016/j.aca.2014.12.018

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A flow injection system incorporated with a polycation-sensitive polymeric membrane electrode in the flow cell is proposed for potentiometric determination of heparin. An external current in nano-ampere scale is continuously applied across the polymeric membrane for controlled release of protamine from the inner filling solution to the sample solution, which makes the electrode membrane regenerate quickly after each measurement. The protamine released at membrane-sample interface is consumed by heparin injected into the flow cell via their strong electrostatic interaction, thus decreasing the measured potential, by which heparin can be detected. Under optimized conditions, a linear relationship between the potential peak height and the concentration of heparin in the sample solution can be obtained in the range of 0.1-2.0 U mL (1), and the detection limit is 0.06 U mL (1). The proposed potentiometric sensing system has been successfully applied to the determination of heparin in undiluted sheep whole blood. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：60 / 65

页数：6

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