New polymorphic markers in the vicinity of the pearl locus on mouse Chromosome 13

被引：7
作者：
Xu, HP
Yanak, BL
Wigler, MH
Gorin, MB
机构：
[1] UNIV PITTSBURGH,INST EYE & EAR,DEPT OPHTHALMOL,PITTSBURGH,PA 15213
[2] COLD SPRING HARBOR LAB,COLD SPRING HARBOR,NY 11724
[3] UNIV PITTSBURGH,DEPT HUMAN GENET,PITTSBURGH,PA 15213
来源：
MAMMALIAN GENOME | 1996年 / 7卷 / 01期
关键词：
D O I：
10.1007/s003359900005
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have used a Mus domesticus/-Mus spretus congenic animal that was selected for retention of Mus spretus DNA around the pearl locus to create a highly polymorphic region suitable for screening new markers. Representation difference analysis (RDA) was performed with either DNA from the congenic animal or C57BL/6J as the driver for subtraction. Four clones were identified, characterized, and converted to PCR-based polymorphic markers. Three of the foul markers equally subdivide a 10-cM interval containing the pearl locus, with the fourth located centromeric to it. These markers have been placed on the mouse genetic map by use of an interspecific backcross panel between Mus domesticus (C57BL/6J) and Mus spretus generated by The Jackson Laboratory.
引用
收藏
页码：16 / 19
页数：4
相关论文
共 11 条
  • [1] GENETIC-ANALYSIS OF THE MOUSE USING INTERSPECIFIC CROSSES
    AVNER, P
    AMAR, L
    DANDOLO, L
    GUENET, JL
    [J]. TRENDS IN GENETICS, 1988, 4 (01) : 18 - 23
  • [2] BUFFER GRADIENT GELS AND S-35 LABEL AS AN AID TO RAPID DNA-SEQUENCE DETERMINATION
    BIGGIN, MD
    GIBSON, TJ
    HONG, GF
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (13): : 3963 - 3965
  • [3] Davis LG., 1986, Basic Methods in Molecular Biology
  • [4] DIETRICH W, 1992, GENETICS, V131, P423
  • [5] LINKAGE OF LOCI AFFECTING A MURINE LIVER PROTEIN AND ARYLSULFATASE-B TO CHROMOSOME-13
    ELLIOTT, RW
    DANIEL, WL
    TAYLOR, BA
    NOVAK, EK
    [J]. JOURNAL OF HEREDITY, 1985, 76 (04) : 243 - 246
  • [6] MOUSE CHROMOSOME-13
    JUSTICE, MJ
    STEPHENSON, DA
    [J]. MAMMALIAN GENOME, 1993, 4 : S192 - S202
  • [7] CLONING THE DIFFERENCES BETWEEN 2 COMPLEX GENOMES
    LISITSYN, N
    LISITSYN, N
    WIGLER, M
    [J]. SCIENCE, 1993, 259 (5097) : 946 - 951
  • [8] MUS SPRETUS-SPECIFIC LINE-1 DNA PROBES APPLIED TO THE CLONING OF THE MURINE PEARL LOCUS
    RIKKE, BA
    PINTO, LH
    GORIN, MB
    HARDIES, SC
    [J]. GENOMICS, 1993, 15 (02) : 291 - 296
  • [9] MAPS FROM 2 INTERSPECIFIC BACKCROSS DNA PANELS AVAILABLE AS A COMMUNITY GENETIC-MAPPING RESOURCE
    ROWE, LB
    NADEAU, JH
    TURNER, R
    FRANKEL, WN
    LETTS, VA
    EPPIG, JT
    KO, MSH
    THURSTON, SJ
    BIRKENMEIER, EH
    [J]. MAMMALIAN GENOME, 1994, 5 (05) : 253 - 274
  • [10] Sambrook J., 1989, MOL CLONING LABORATO, V2nd
12