A highly sensitive and selective detection of Hg(II) in 100% aqueous solution with fluorescent labeled dimerized Cys residues

被引：88

作者：

Joshi, Bishnu Prasad ^{[1
]}

Lohani, Chuda Raj ^{[1
]}

Lee, Keun-Hyeung ^{[1
]}

机构：

[1] Inha Univ, Dept Chem, Bioorgan Chem Lab, Inchon 402751, South Korea

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2010年 / 8卷 / 14期

关键词：

4 DANSYL FLUOROPHORES; TURN-ON; METAL-IONS; PEPTIDE SENSOR; MERCURIC ION; BEARING; CHEMODOSIMETER; CHEMOSENSOR; PROTEINS; CELLS;

D O I：

10.1039/b925744f

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A simple design of a ratiometric fluorescent sensor for detecting Hg(II) ion in 100% aqueous solution was demonstrated, based on the structure of dimerized Cys residues with two dansyl fluorophores. The sensor highly sensitively and selectively detected mercury ion (K-d = 41 nM) in 100% aqueous solution via a turn-on and ratiometric response. The sensor showed no interferences of other metal ions and satisfied for monitoring the maximum allowable level (2 ppb) of mercury ion in drinking water demanded by EPA via a turn-on response.

引用

页码：3220 / 3226

页数：7

共 50 条

[1]

[Anonymous], 2004, Guidelines for Drinking Water Quality

[2] A double-functionalized cyclen with carbamoyl and dansyl groups (cyclen=1,4,7,10-tetraazacyclododecane):: A selective fluorescent probe for Y3+ and La3+ [J].

Aoki, S ;

Kawatani, H ;

Goto, T ;

Kimura, E ;

Shiro, M .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (06) :1123-1132

[3] Dendrimers as fluorescent sensors with signal amplification [J].

Balzani, V ;

Ceroni, P ;

Gestermann, S ;

Kauffmann, C ;

Gorka, M ;

Vögtle, F .

CHEMICAL COMMUNICATIONS, 2000, (10) :853-854

[4] Ionization-reactivity relationships for cysteine thiols in polypeptides [J].

Bulaj, G ;

Kortemme, T ;

Goldenberg, DP .

BIOCHEMISTRY, 1998, 37 (25) :8965-8972

[5] A general strategy to convert the MerR family proteins into highly sensitive and selective fluorescent biosensors for metal ions [J].

Chen, P ;

He, CA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (03) :728-729

[6] Selective recognition of metal ions by metalloregulatory proteins [J].

Chen, Peng R. ;

He, Chuan .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2008, 12 (02) :214-221

[7] Bis(2-pyridyl)-substituted boratriazaindacene as an NIR-emitting chemosensor for Hg(II) [J].

Coskun, Ali ;

Yilmaz, M. Deniz ;

Akkaya, Engin U. .

ORGANIC LETTERS, 2007, 9 (04) :607-609

[8] A selective, ratiometric fluorescent sensor for Pb2+ [J].

Deo, S ;

Godwin, HA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (01) :174-175

[9]

*EPA OFF WAT, 2001, EPA823F01011 OFF WAT

[10] SOLID-PHASE PEPTIDE-SYNTHESIS UTILIZING 9-FLUORENYLMETHOXYCARBONYL AMINO-ACIDS [J].

FIELDS, GB ;

NOBLE, RL .

INTERNATIONAL JOURNAL OF PEPTIDE AND PROTEIN RESEARCH, 1990, 35 (03) :161-214

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