Amifostine Protection Against Induced DNA Damage in γ-Irradiated Escherichia coli Cells Depend on recN DNA Repair Gene Product Activity

被引：6
|
作者：
Almeida, Eliseo [2 ]
Luis Fuentes, Jorge [1 ]
Cuetara, Elizabeth [3 ]
Prieto, Elio [4 ]
Llagostera, Montserrat [5 ,6 ]
机构：
[1] Univ Ind Santander, Lab Microbiol & Mutagenesis Ambiental, Escuela Biol, Fac Ciencias, Bucaramanga, Colombia
[2] Ctr Aplicac Tecnol & Desarrollo Nucl CEADEN, Dept Radiobiol, Havana, Cuba
[3] Inst Nacl Oncol & Radiobiol, Lab Farmacol Clin & Expt, Havana, Cuba
[4] Univ Abierta Interamer, Ctr Altos Estudios Ciencias Salud, RA-1270 Buenos Aires, DF, Argentina
[5] Univ Autonoma Barcelona, Dept Genet & Microbiol, Bellaterra 08193, Spain
[6] Univ Autonoma Barcelona, CReSA, IRTA, E-08193 Barcelona, Spain
来源：
ENVIRONMENTAL TOXICOLOGY | 2010年 / 25卷 / 02期
关键词：
amifostine; Escherichia coli; gamma-rays; recN gene; SOS response; DOUBLE-STRAND BREAKS; PHOSPHORIBOSYL TRANSFERASE LOCUS; SOS CHROMOTEST; V79; CELLS; INDUCED MUTATIONS; WR-2721; RADIATION; RADIOPROTECTOR; RECOMBINATION; GENOTOXICITY;
D O I：
10.1002/tox.20483
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Amifostine is the most effective radioprotector known and the only one accepted for clinical use in cancer radiotherapy. In this work, the antigenotoxic effect of amifostine against gamma-rays was studied in Escherichia coli cells deficient in DNA damage repair activities. Assays of irradiated cells treated with amifostine showed that the drug reduced the genotoxicity induced by radiation in E. coli wild-type genotypes and in uvr, recF, recB, recB-recC-recF mutant strains, but not in recN defective cells. Thus, the mechanism of DNA protection by amifostine against gamma-radiation-induced genotoxicity appears to involve participation of the RecN protein that facilitates repair of DNA double-strand breaks. The results are discussed in relation to amifostine's chemopreventive potential. (C) 2009 Wiley Periodicals, Inc. Environ Toxicol 25: 130-136, 2010.
引用
收藏
页码：130 / 136
页数：7
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