DbClustal: rapid and reliable global multiple alignments of protein sequences detected by database searches

被引：150

作者：

Thompson, JD ^{[1
]}

Plewniak, F ^{[1
]}

Thierry, JC ^{[1
]}

Poch, O ^{[1
]}

机构：

[1] ULP, INSERM, CNRS, Inst Genet & Biol Mol & Cellulaire,Lab Biol & Gen, F-67404 Illkirch Graffenstaden, France

来源：

NUCLEIC ACIDS RESEARCH | 2000年 / 28卷 / 15期

关键词：

D O I：

10.1093/nar/28.15.2919

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DbClustal addresses the important problem of the automatic multiple alignment of the top scoring full-length sequences detected by a database homology search. By combining the advantages of both local and global alignment algorithms into a single system, DbClustal is able to provide accurate global alignments of highly divergent, complex sequence sets. Local alignment information is incorporated into a ClustalW global alignment in the form of a list of anchor points between pairs of sequences. The method is demonstrated using anchors supplied by the Blast post-processing program, Ballast. The rapidity and reliability of DbClustal have been demonstrated using the recently annotated Pyrococcus abyssi proteome where the number of alignments with totally misaligned sequences was reduced from 20% to <2%. A web site has been implemented proposing BlastP database searches with automatic alignment of the top hits by DbClustal.

引用

页码：2919 / 2926

页数：8

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