Promotion of attachment of human bone marrow stromal cells by CCN2

被引：23

作者：

Ono, Mitsuaki

Kubota, Satoshi

Fujisawa, Takuo

Sonoyama, Wataru

Kawaki, Harumi

Akiyama, Kentaro

Oshima, Masamitsu

Nishida, Takashi

Yoshida, Yasuhlro

Suzuki, Kazuomi

Takigawa, Masaharu

Kuboki, Takuo

机构：

[1] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Oral & Maxillofacial Rehabil, Okayama 7008525, Japan

[2] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Biochem & Mol Dent, Okayama 7008525, Japan

[3] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Biomat, Okayama 7008525, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 357卷 / 01期

基金：

日本学术振兴会;

关键词：

bone marrow stromal cell; CCN32/connective tissue growth factor; cell attachment; p38; MAPK; hydroxyapatite; TISSUE GROWTH-FACTOR; VASCULAR ENDOTHELIAL-CELLS; CHONDROCYTE-SPECIFIC GENE; MC3T3-E1; OSTEOBLASTS; STEM-CELLS; IN-VITRO; DIFFERENTIATION; PROLIFERATION; CTGF/HCS24; PRECURSORS;

D O I：

10.1016/j.bbrc.2007.03.052

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cell attachment is a crucial step in tissue regeneration. In this study, human bone marrow stromal cells (hBMSCs) were isolated, and the effects of CCN2 on their attachment were examined. CCN2 significantly enhanced the hBMSC attachment, and this enhanced cell attachment was mainly regulated by the C-terminal module of CCN2. This enhancement was negated by the anti-integrin alpha(v)beta(3) antibody and p38 MAPK inhibitor, and phosphorylation of p38 MAPK was detected upon the enhanced cell attachment mediated by CCN2. We thus conclude that CCN2 enhances hBMSC attachment via integrin-p38 MAPK signal pathway. Enhanced hBMSC attachment on hydroxyapatite plates by CCN2 further indicated the utility of CCN2 in bone regeneration. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：20 / 25

页数：6

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