Improved hepatic MRP2/ABCC2 transport activity in LPS-induced cholestasis by aquaporin-1 gene transfer

被引：9
作者：
Marrone, Julieta [1 ]
Tocchetti, Guillermo N. [1 ]
Danielli, Mauro [1 ]
Mottino, Aldo D. [1 ]
Marinelli, Raul A. [1 ]
机构：
[1] Univ Nacl Rosario, Inst Fisiol Expt, CONICET, Fac Ciencias Bioquim & Farmaceut, RA-2000 Rosario, Santa Fe, Argentina
来源：
BIOCHIMIE | 2019年 / 165卷
关键词：
Aquaporin-1; Cholestasis triggered by LPS; Biliary glutathione excretion; Multidrug resistance-associated protein-2; Adenoviral gene transfer; LIVER; MRP2; LIPOPOLYSACCHARIDE; LOCALIZATION; GLUTATHIONE; EXPRESSION; PROTEIN-2; EXCRETION; RATS;
D O I：
10.1016/j.biochi.2019.07.027
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Multidrug resistance-associated protein 2 (MRP2/ABCC2), a hepatocyte canalicular transporter involved in bile secretion, is downregulated in cholestasis triggered by lipopolysaccharide. The human aquaporin-1 (hAQP1) adenovirus-mediated gene transfer to liver improves cholestasis by incompletely defined mechanisms. Here we found that hAQP1 did not affect MRP2/ABCC2 expression, but significantly increased its transport activity assessed in situ with endogenous and exogenous substrates, likely by a hAQP1-induced increase in canalicular membrane cholesterol amount. Our results suggest that hAQP1-induced MRP2/ABCC2 activation contributes to the cholestasis improvement. (C) 2019 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.
引用
收藏
页码：179 / 182
页数：4
相关论文
共 18 条
[1]  
Bhogal Harjit K, 2013, Front Biosci (Elite Ed), V5, P87
[2]   LPS-induced downregulation of MRP2 and BSEP in human liver is due to a posttranscriptional process [J].
Elferink, MGL ;
Olinga, P ;
Draaisma, AL ;
Merema, MT ;
Faber, KN ;
Slooff, MJH ;
Meijer, DKF ;
Groothuis, GMM .
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2004, 287 (05) :G1008-G1016
[3]   Principles of hepatic organic anion transporter regulation during cholestasis, inflammation and liver regeneration [J].
Geier, Andreas ;
Wagner, Martin ;
Dietrich, Christoph G. ;
Trauner, Michael .
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2007, 1773 (03) :283-308
[4]   Differential Effects of Membrane Cholesterol Content on the Transport Activity of Multidrug Resistance-Associated Protein 2 (ABCC2) and of the Bile Salt Export Pump (ABCB11) [J].
Guyot, Christelle ;
Hofstetter, Lia ;
Stieger, Bruno .
MOLECULAR PHARMACOLOGY, 2014, 85 (06) :909-920
[5]   Effects of lipopolysaccharide on the biliary excretion of bile acids and organic anions in rats [J].
Hojo, M ;
Sano, N ;
Takikawa, H .
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, 2003, 18 (07) :815-821
[6]   LPS induces the TNF-α-mediated downregulation of rat liver aquaporin-8:: role in sepsis-associated cholestasis [J].
Lehmann, Guillermo L. ;
Carreras, Flavia I. ;
Soria, Leandro R. ;
Gradilone, Sergio A. ;
Marinelli, Raul A. .
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2008, 294 (02) :G567-G575
[7]  
LOWRY OH, 1951, J BIOL CHEM, V193, P265
[8]   Adenoviral transfer of human aquaporin-1 gene to rat liver improves bile flow in estrogen-induced cholestasis [J].
Marrone, J. ;
Lehmann, G. L. ;
Soria, L. R. ;
Pellegrino, J. M. ;
Molinas, S. ;
Marinelli, R. A. .
GENE THERAPY, 2014, 21 (12) :1058-1064
[9]   Adenovirus-mediated human aquaporin-1 expression in hepatocytes improves lipopolysaccharide-induced cholestasis [J].
Marrone, Julieta ;
Danielli, Mauro ;
Gaspari, Cesar I. ;
Marinelli, Raul A. .
IUBMB LIFE, 2017, 69 (12) :978-984
[10]   Hepatic Gene Transfer of Human Aquaporin-1 Improves Bile Salt Secretory Failure in Rats With Estrogen-Induced Cholestasis [J].
Marrone, Julieta ;
Soria, Leandro R. ;
Danielli, Mauro ;
Lehmann, Guillermo L. ;
Cecilia Larocca, Maria ;
Marinelli, Raul A. .
HEPATOLOGY, 2016, 64 (02) :535-548
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