Expression of the VRK (vaccinia-related kinase) gene family of p53 regulators in murine hematopoletic development

被引:46
作者
Vega, FM
Gonzalo, P
Gaspar, ML
Lazo, PA [1 ]
机构
[1] Univ Salamanca, CSIC, Ctr Invest Canc, Inst Biol Mol & Celular Canc, E-37007 Salamanca, Spain
[2] Inst Salud Carlos III, Ctr Nacl Microbiol, Unidad Inmunobiol, E-28220 Majadahonda, Spain
关键词
VRK1; VRK2; VRK3; p53; Ser-Thr kinase; mouse development; liver; hematopoiesis;
D O I
10.1016/S0014-5793(03)00501-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The vaccinia-related kinase (VRK) proteins are a new group of three Ser-Thr kinases in the human kinome. VRK proteins are upstream regulators of several transcription factors. VRK1 phosphorylates p53 in Thr-18 within the region of binding to mdm2 preventing their interaction. The tissue distribution of three genes is still largely unknown. In the present report the expression of these genes was analyzed during murine hematopoietic development. The three genes are expressed in fetal liver and peripheral blood, with higher levels between days 11.5 and 13.5, a time when there is a massive expansion of liver cells, and thereafter their expression falls significantly. VRK genes are expressed, particularly at mid-gestation, in embryo thymus and spleen, but in adult thymus and spleen their levels are very low. VRK2 is expressed at lower levels than VRK1 and VRK3 in the mouse embryo. VRK genes play a role during embryonic development of hematopoiesis. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
引用
收藏
页码:176 / 180
页数:5
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