Iron (Fe) and sulfur (S) interaction was studied in bread and durum wheat by using radiotracers (Fe-59 and S-35). The results showed a higher uptake of S-35 under Fe+ than Fe- which provides irrefutable evidence for the role of Fe in determining plant S uptake particularly under S deficiency. Under Fe deficiency, the Fe-59 uptake was higher under the S2 than S1 and S0 condition, which may be attributed to a favorable regulation of phytosiderophores biosynthesis by S. This suggests that Fe and S synergistically interact and may regulate their respective uptake by inducing SULTR1; 1, high affinity sulphate transporters.