Purification and characterization of a fucoidan from the brown algae Macrocystis pyrifera and the activity of enhancing salt-stress tolerance of wheat seedlings

A fuciodan (Mw = 11.1 kDa) was obtained and purified from Macrocystis pyrifera (MPF). MPF was an acid heteropolysaccharide including fucose, mannose, xylose, galactose, rhamnose, glucuronic acid, and glucose in a molar ratio of 3.1:1.0:0.86:0.63:0.25:0.33:0.11. Sulfate content in MPF was 28.6%, and the molar ratio of fucose to sulfate (Fuc:Sa(4)(2-)) was 1.0:0.58. The structure of MPF was mainly consist of repeating -> 3)-beta-L-Fucp (2SO(3)(-))-(1 -> and -> 4)-beta-D-Xylp-(1 -> 3)-beta-L-Fucp(2SO(3)(-))-(1 -> and with alpha-L-Fucp-(1 -> and -> 6)-alpha-D-Galp-(1 -> in branches. Moreover, the effects of different MPF concentrations on plant salt tolerance were investigated. The results indicated that MPF could improve the salt tolerance of wheat seedlings. Among the five concentrations (0.05,0.1,0.5, Land 2 mg/ml), 0.5 and 1 mg/ml MPF were optimal for effective plant salt-resistance activity. These results suggested that MPT extracted from brown seaweed show potential as plant stimulators that may be used to improve salt resistance of plants. (C) 2021 Published by Elsevier B.V.