Impact of human myelin on the maturation and function of human monocyte-derived dendritic cells

被引：8

作者：

Gredler, Viktoria ^{[1
]}

Ebner, Susanne ^{[2
]}

Schanda, Kathrin ^{[1
]}

Forstner, Markus ^{[2
]}

Berger, Thomas ^{[1
]}

Romani, Nikolaus ^{[2
]}

Reindl, Markus ^{[1
]}

机构：

[1] Innsbruck Med Univ, Dept Clin Neurol, A-6020 Innsbruck, Austria

[2] Innsbruck Med Univ, Dept Dermatol & Venereol, A-6020 Innsbruck, Austria

来源：

CLINICAL IMMUNOLOGY | 2010年 / 134卷 / 03期

关键词：

Dendritic cells; Multiple sclerosis; Human; Myelin; Neuroimmunology; MULTIPLE-SCLEROSIS; AUTOIMMUNE ENCEPHALOMYELITIS; MEDICAL PROGRESS; HUMAN BLOOD; GENERATION; BETA; PHAGOCYTOSIS; PATHOGENESIS; INFLAMMATION; MACROPHAGES;

D O I：

10.1016/j.clim.2009.11.003

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Macrophages and dendritic cells (DC) play an important role in the immunopathology of multiple sclerosis. We analyzed the impact of human myelin on monocyte-derived DC and describe their immunostimulatory capacity. Celts were grown on myelin and stimulated with LPS or a defined maturation cocktail. DC activation was analyzed by the expression of cell surface markers and the secretion of cytokines and chemokines. The immunostimulatory capacity of DC was assessed by allogeneic mixed-leukocyte reactions via proliferation. Additionally, their ability to bias T cells towards Th1, Th17 or Treg differentiation was investigated. We found that phagocytosis of myelin impaired the activation of DC, displayed by an impaired ability to stimulate allogeneic T cells, an increased production of TGF-beta 1 and a diminished upregulation of CCR7 but did not affect the differentiation into T helper cell subsets. We hypothesize that myelin influences DC activation and plays a pivotal role in balancing immunity and tolerance. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：296 / 304

页数：9

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