Electrochemical Aptasensor Based on Polycarboxylic Macrocycle Modified with Neutral Red for Aflatoxin B1 Detection

被引：83

作者：

Evtugyn, Gennady ^{[1
]}

Porfireva, Anna ^{[1
]}

Stepanova, Veronika ^{[1
]}

Sitdikov, Rusal ^{[2
]}

Stoikov, Ivan ^{[2
]}

Nikolelis, Dimitrios ^{[3
]}

Hianik, Tibor ^{[4
]}

机构：

[1] Kazan Fed Univ, Dept Analyt Chem, Kazan 420008, Russia

[2] Kazan Fed Univ, Dept Organ Chem, Kazan 420008, Russia

[3] Univ Athens, Dept Chem, Environm Chem Lab, GR-15771 Athens, Greece

[4] Comenius Univ, Dept Nucl Phys & Biophys, Bratislava 84248, Slovakia

来源：

ELECTROANALYSIS | 2014年 / 26卷 / 10期

关键词：

Aptasensor; Aflatoxin B1; Thiacalix[4]arene; DNA Aptamer; Voltammetry; Electrochemical impedance spectroscopy; IMPEDIMETRIC APTASENSOR; ULTRASENSITIVE APTASENSOR; POLY(NEUTRAL RED); RAPID DETECTION; THROMBIN; IMMUNOSENSOR; B-1; OCHRATOXIN; MYCOTOXINS; BIOSENSOR;

D O I：

10.1002/elan.201400328

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Novel electrochemical aptasensors based on glassy carbon electrodes modified with electropolymerized Neutral red and polycarboxylated macrocyclic ligands onto which the DNA aptamers were covalently attached have been developed for detection of Aflatoxin B1 (AFB1). The interaction with an analyte resulted in the decrease of the cathodic peak current of the probe measured by CV and in the increase of the electron transfer resistance determined by EIS. The limit of detection was found to be 0.1 nM for CV and 0.05 nM for EIS methods, respectively. The aptasensor makes it possible to detect AFB1 in peanuts, cashew nuts, white wine and soy sauce with a recovery of 85-100%.

引用

页码：2100 / 2109

页数：10

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