SNAREpins: Minimal machinery for membrane fusion

被引：2030

作者：

Weber, T ^{[1
]}

Zemelman, BV ^{[1
]}

McNew, JA ^{[1
]}

Westermann, B ^{[1
]}

Gmachl, M ^{[1
]}

Parlati, F ^{[1
]}

Sollner, TH ^{[1
]}

Rothman, JE ^{[1
]}

机构：

[1] Mem Sloan Kettering Canc Ctr, Cellular Biochem & Biophys Program, New York, NY 10021 USA

来源：

CELL | 1998年 / 92卷 / 06期

基金：

美国国家卫生研究院; 英国医学研究理事会;

关键词：

D O I：

10.1016/S0092-8674(00)81404-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recombinant v- and t-SNARE proteins reconstituted into separate lipid bilayer vesicles assemble into SNARE-pins-SNARE complexes linking two membranes. This leads to spontaneous fusion of the docked membranes at physiological temperature. Docked unfused intermediates can accumulate at lower temperatures and can fuse when brought to physiological temperature. A supply of unassembled v-and t-SNAREs is needed for these intermediates to form, but not for the fusion that follows. These data imply that SNARE-pins are the minimal machinery for cellular membrane fusion.

引用

页码：759 / 772

页数：14

共 81 条

[21] Syntaxin 6 functions in trans-Golgi network vesicle trafficking [J].

Davanger, S ;

Bock, JB ;

Klumperman, J ;

Scheller, RH .

MOLECULAR BIOLOGY OF THE CELL, 1997, 8 (07) :1261-1271

[22] How do viruses enter cells? The HIV coreceptors teach us a lesson of complexity [J].

Dimitrov, DS .

CELL, 1997, 91 (06) :721-730

[23]

DUNN B, 1993, NATURE, V362, P53

[24] SYNAPTOBREVIN BINDING TO SYNAPTOPHYSIN - A POTENTIAL MECHANISM FOR CONTROLLING THE EXOCYTOTIC FUSION MACHINE [J].

EDELMANN, L ;

HANSON, PI ;

CHAPMAN, ER ;

JAHN, R .

EMBO JOURNAL, 1995, 14 (02) :224-231

[25] Structural changes are associated with soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor complex formation [J].

Fasshauer, D ;

Otto, H ;

Eliason, WK ;

Jahn, R ;

Brunger, AT .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (44) :28036-28041

[26] A structural change occurs upon binding of syntaxin to SNAP-25 [J].

Fasshauer, D ;

Bruns, D ;

Shen, B ;

Jahn, R ;

Brunger, AT .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (07) :4582-4590

[27] A RAT-BRAIN SEC1 HOMOLOG RELATED TO ROP AND UNC18 INTERACTS WITH SYNTAXIN [J].

GARCIA, EP ;

GATTI, E ;

BUTLER, M ;

BURTON, J ;

DECAMILLI, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (06) :2003-2007

[28] In vivo labeling of over-expressed recombinant proteins in E-coli [J].

Giovane, C ;

Schwalbach, G ;

Weiss, E .

BIOTECHNIQUES, 1997, 22 (05) :796-798

[29] Botulinum neurotoxin light chains inhibit both Ca2+-induced and CTP analogue-induced catecholamine release from permeabilised adrenal chromaffin cells [J].

Glenn, DE ;

Burgoyne, RD .

FEBS LETTERS, 1996, 386 (2-3) :137-140

[30] Structure and conformational changes in NSF and its membrane receptor complexes visualized by quick-freeze/deep-etch electron microscopy [J].

Hanson, PI ;

Roth, R ;

Morisaki, H ;

Jahn, R ;

Heuser, JE .

CELL, 1997, 90 (03) :523-535

← 1 2 3 4 5 6 7 8 9 →