Combined use of eBeam irradiation and the potential probiotic Lactobacillus rhamnosus Vahe for control of foodborne pathogen Klebsiella pneumoniae

被引：17

作者：

Balayan, Marine H. ^{[1
]}

Pepoyan, Astghik Z. ^{[1
,2
]}

Manvelyan, Anahit M. ^{[1
]}

Tsaturyan, Vardan V. ^{[3
]}

Grigoryan, Bagrat ^{[4
]}

Abrahamyan, Arusyak ^{[5
]}

Chikindas, Michael L. ^{[6
,7
]}

机构：

[1] Armenian Natl Agr Univ, Dept Food Safety & Biotechnol, Teryan 74, Yerevan 0009, Armenia

[2] Int Assoc Human & Anim Hlth Improvement, Azatutyan 11, Yerevan 0037, Armenia

[3] Yerevan State Med Univ Mkhitar Heratsi, Koryun 2, Yerevan 0025, Armenia

[4] CANDLE Synchrotron Res Inst, 31 Acharyan Str, Yerevan 0040, Armenia

[5] Univ Guelph, Canadian Ctr DNA Barcoding, Guelph, ON N1G 2W1, Canada

[6] Southern Fed Univ, Acad Biol & Biotechnol, Stachki Ave 194-1, Rostov Na Donu 344090, Russia

[7] Rutgers State Univ, Hlth Promoting Nat Lab, 65 Dudley Rd, New Brunswick, NJ 08901 USA

来源：

ANNALS OF MICROBIOLOGY | 2019年 / 69卷 / 13期

关键词：

Lactobacillus rhamnosus Vahe; Cell viability; Antimicrobial activity; Probiotic; COMMENSAL ESCHERICHIA-COLI; GROWTH;

D O I：

10.1007/s13213-019-01522-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Purpose The implementation of electron beam radiation coupled with the use of probiotics is one of the newest food processing technologies that may be used to ensure food safety and improve shelf life of food products. The purpose of this study was to evaluate the effect of 50-150-Gy electron beam irradiation on the antimicrobial activity of the putative probiotic strain Lactobacillus rhamnosus Vahe. Methods Low-dose electron beam irradiation of lactobacilli cells was performed using the Advanced Research Electron Accelerator Laboratory's electron accelerator, and the agar well diffusion method and Verhulst logistic function were used to evaluate the effect of radiation on anti-Klebsiella pneumoniae activity of the cell free supernatant of L. rhamnosus Vahe cells in vitro. Results Our results suggest that 50-150-Gy electron beam irradiation decreases the viability of the investigated lactobacilli, but does not significantly change the probiotic's activity against K. pneumoniae. Conclusions Results indicate that the combined use of irradiation and L. rhamnosus Vahe might be suggested for non-thermal food sterilizing technologies.

引用

页码：1579 / 1582

页数：4

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