Identification and Functional Characterization of a Novel Insecticidal Decapeptide from the Myrmicine Ant Manica rubida

被引:21
作者
Heep, John [1 ]
Skaljac, Marisa [1 ]
Grotmann, Jens [1 ]
Kessel, Tobias [1 ]
Seip, Maximilian [1 ]
Schmidtberg, Henrike [2 ]
Vilcinskas, Andreas [1 ,2 ]
机构
[1] Fraunhofer Inst Mol Biol & Appl Ecol IME, Branch Bioresources, Winchesterstr 2, D-35394 Giessen, Germany
[2] Justus Liebig Univ Giessen, Inst Insect Biotechnol, Heinrich Buff Ring 26-32, D-35392 Giessen, Germany
关键词
mass spectrometry; LC-MS; Formicidae; Myrmicinae; Myrmica rubra; venom gland; bioinsecticide; antimicrobial peptide; aphids; Acyrthosiphon pisum; SPIDER-VENOM PEPTIDES; PEACH-POTATO APHID; ANTIMICROBIAL PEPTIDES; HYMENOPTERA-FORMICIDAE; ACYRTHOSIPHON-PISUM; EXPRESSION; DIVERSITY; PROTEIN; TOXINS; GENE;
D O I
10.3390/toxins11100562
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Ant venoms contain many small, linear peptides, an untapped source of bioactive peptide toxins. The control of agricultural insect pests currently depends primarily on chemical insecticides, but their intensive use damages the environment and human health, and encourages the emergence of resistant pest populations. This has promoted interest in animal venoms as a source of alternative, environmentally-friendly bio-insecticides. We tested the crude venom of the predatory ant, Manica rubida, and observed severe fitness costs in the parthenogenetic pea aphid (Acyrthosiphon pisum), a common agricultural pest. Therefore, we explored the M. rubida venom peptidome and identified a novel decapeptide U-MYRTX-MANr1 (NH2-IDPKVLESLV-CONH2) using a combination of Edman degradation and de novo peptide sequencing. Although this myrmicitoxin was inactive against bacteria and fungi, it reduced aphid survival and reproduction. Furthermore, both crude venom and U-MYRTX-MANr1 reversibly paralyzed injected aphids and induced a loss of body fluids. Components of M. rubida venom may act on various biological targets including ion channels and hemolymph coagulation proteins, as previously shown for other ant venom toxins. The remarkable insecticidal activity of M. rubida venom suggests it may be a promising source of additional bio-insecticide leads.
引用
收藏
页数:17
相关论文
共 76 条
[1]   A method of computing the effectiveness of an insecticide [J].
Abbott, WS .
JOURNAL OF ECONOMIC ENTOMOLOGY, 1925, 18 :265-267
[2]   Combined Peptidomic and Proteomic Analysis of Electrically Stimulated and Manually Dissected Venom from the South American Bullet Ant Paraponera clavata [J].
Aili, Samira R. ;
Touchard, Axel ;
Petitclerc, Frederic ;
Dejean, Alain ;
Orivel, Jerome ;
Padula, Matthew P. ;
Escoubas, Pierre ;
Nicholson, Graham M. .
JOURNAL OF PROTEOME RESEARCH, 2017, 16 (03) :1339-1351
[3]   Diversity of peptide toxins from stinging ant venoms [J].
Aili, Samira R. ;
Touchard, Axel ;
Escoubas, Pierre ;
Padula, Matthew P. ;
Orivel, Jerome ;
Dejean, Alain ;
Nicholson, Graham M. .
TOXICON, 2014, 92 :166-178
[4]   CONTINUOUS REARING OF PEA APHID, ACYRTHOSIPHON PISUM, HOMOPTERA-APHIDIDAE ON A HOLIDIC DIET [J].
AKEY, DH ;
BECK, SD .
ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA, 1971, 64 (02) :353-&
[5]   Wounding-mediated gene expression and accelerated viviparous reproduction of the pea aphid Acyrthosiphon pisum [J].
Altincicek, B. ;
Gross, J. ;
Vilcinskas, A. .
INSECT MOLECULAR BIOLOGY, 2008, 17 (06) :711-716
[6]   Sequence-specific determination of protein and peptide concentrations by absorbance at 205 nm [J].
Anthis, Nicholas J. ;
Clore, G. Marius .
PROTEIN SCIENCE, 2013, 22 (06) :851-858
[7]   Eight principles of integrated pest management [J].
Barzman, Marco ;
Barberi, Paolo ;
Birch, A. Nicholas E. ;
Boonekamp, Piet ;
Dachbrodt-Saaydeh, Silke ;
Graf, Benno ;
Hommel, Bernd ;
Jensen, Jens Erik ;
Kiss, Jozsef ;
Kudsk, Per ;
Lamichhane, Jay Ram ;
Messean, Antoine ;
Moonen, Anna-Camilla ;
Ratnadass, Alain ;
Ricci, Pierre ;
Sarah, Jean-Louis ;
Sattin, Maurizio .
AGRONOMY FOR SUSTAINABLE DEVELOPMENT, 2015, 35 (04) :1199-1215
[8]   The evolution of insecticide resistance in the peach potato aphid, Myzus persicae [J].
Bass, Chris ;
Puinean, Alin M. ;
Zimmer, Christoph T. ;
Denholm, Ian ;
Field, Linda M. ;
Foster, Stephen P. ;
Gutbrod, Oliver ;
Nauen, Ralf ;
Slater, Russell ;
Williamson, Martin S. .
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2014, 51 :41-51
[9]   Toxin delivery by the coat protein of an aphid-vectored plant virus provides plant resistance to aphids [J].
Bonning, Bryony C. ;
Pal, Narinder ;
Liu, Sijun ;
Wang, Zhaohui ;
Sivakumar, S. ;
Dixon, Philip M. ;
King, Glenn F. ;
Miller, W. Allen .
NATURE BIOTECHNOLOGY, 2014, 32 (01) :102-+
[10]   Profiling the venom gland transcriptome of Tetramorium bicarinatum (Hymenoptera: Formicidae): The first transcriptome analysis of an ant species [J].
Bouzid, Wafa ;
Klopp, Christophe ;
Verdenaud, Marion ;
Ducancel, Frederic ;
Vetillard, Angelique .
TOXICON, 2013, 70 :70-81