Microbiological Evaluation and Sperm DNA Fragmentation in Semen Samples of Patients Undergoing Fertility Investigation

被引：26

作者：

Pagliuca, Chiara ^{[1
]}

Cariati, Federica ^{[2
]}

Bagnulo, Francesca ^{[3
]}

Scaglione, Elena ^{[1
,4
]}

Carotenuto, Consolata ^{[1
]}

Farina, Fabrizio ^{[5
]}

D'Argenio, Valeria ^{[2
,6
]}

Carraturo, Francesca ^{[1
]}

D'Aprile, Paola ^{[1
]}

Vitiello, Mariateresa ^{[1
]}

Strina, Ida ^{[3
,7
]}

Alviggi, Carlo ^{[3
,7
,8
]}

Colicchio, Roberta ^{[1
]}

Tomaiuolo, Rossella ^{[9
]}

Salvatore, Paola ^{[1
,2
,10
]}

机构：

[1] Univ Naples Federico II, Dept Mol Med & Med Biotechnol, Via S Pansini 5, I-80131 Naples, Italy

[2] CEINGE Biotecnol Avanzate, Via G Salvatore 486, I-80145 Naples, Italy

[3] AOU Policlin Federico II, Fertil Unit, Maternal Child Dept, I-80131 Naples, Italy

[4] Univ Naples Federico II, CeSMA Ctr Serv Metrol & Tecnol Avanzati, Corso Nicolangelo Protopisani, I-80146 Naples, Italy

[5] Univ Sannio, Dept Law Econ Management & Quantitat Methods, I-82100 Benevento, Italy

[6] San Raffaele Open Univ, Dept Human Sci & Qual Life Promot, Via Val Cannuta 247, I-00166 Rome, Italy

[7] Univ Naples Federico II, Dept Neurosci Reprod Sci & Odontostomatol, I-80138 Naples, Italy

[8] CNR, Endocrinol & Expt Oncol Inst IEOS, I-80131 Naples, Italy

[9] Univ Vita Salute San Raffaele, I-20132 Milan, Italy

[10] Univ Naples Federico II, Task Force Microbiome Studies, I-80138 Naples, Italy

来源：

GENES | 2021年 / 12卷 / 05期

关键词：

urogenital infections; microbiological evaluation; sperm DNA fragmentation; male infertility; HUMAN SPERMATOZOA; CHROMATIN-STRUCTURE; OXIDATIVE STRESS; MALE-INFERTILITY; DAMAGE; INFECTION; FERTILIZATION; INFLAMMATION; PROSTATITIS; INTEGRITY;

D O I：

10.3390/genes12050654

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Fifteen percent of male infertility is associated with urogenital infections; several pathogens are able to alter the testicular and accessory glands' microenvironment, resulting in the impairment of biofunctional sperm parameters. The purpose of this study was to assess the influence of urogenital infections on the quality of 53 human semen samples through standard analysis, microbiological evaluation, and molecular characterization of sperm DNA damage. The results showed a significant correlation between infected status and semen volume, sperm concentration, and motility. Moreover, a high risk of fragmented sperm DNA was demonstrated in the altered semen samples. Urogenital infections are often asymptomatic and thus an in-depth evaluation of the seminal sample can allow for both the diagnosis and therapy of infections while providing more indicators for male infertility management.

引用

页数：12

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[1] Sperm DNA damage assessment: a test whose time has come [J].