Anticoagulants used in plasma collection affect adipokine multiplexed measurements

被引：4

作者：

Allione, Alessandra ^{[1
,2
]}

Di Gaetano, Cornelia ^{[1
,2
]}

Dani, Nadia ^{[3
]}

Barberio, Davide ^{[3
]}

Sieri, Sabina ^{[4
]}

Krogh, Vittorio ^{[4
]}

Matullo, Giuseppe ^{[1
,2
]}

机构：

[1] Human Genet Fdn HuGeF, Via Nizza 52, I-10126 Turin, Italy

[2] Univ Turin, Dept Med Sci, Via Santena 19, I-10100 Turin, Italy

[3] Bioclarma Srl, Via Nizza 52, I-10126 Turin, Italy

[4] IRCCS Fdn, Ist Nazl Tumori, Via Venezian, I-20133 Milan, Italy

来源：

CYTOKINE | 2016年 / 80卷

关键词：

Adipokines; Anticoagulants; Cytokines; Microbead assay; Plasma; HEALTHY-SUBJECTS; CYTOKINE LEVELS; DISEASE; SAMPLES; ASSAYS; PROFILES; SERUM; KITS;

D O I：

10.1016/j.cyto.2016.03.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Obesity is an important health problem worldwide. Adipose tissue acts as an endocrine organ that secretes various bioactive substances, called adipokines, including pro-inflammatory biomarkers such as TNF-alpha, IL-6, leptin and C-reactive protein (CRP) and anti-inflammatory molecules such as adiponectin. The deregulated production of adipokines in obesity is linked to the pathogenesis of various disease processes and monitoring their variation is critical to understand metabolic diseases. The aim of this study was to determine the plasma concentration of adipokines in healthy subjects by multiplexed measurements and the effect of anticoagulants on their levels. Plasma samples from 10 healthy donors were collected in two different anticoagulants (sodium citrate or heparin). All markers, excluding TNF-alpha, showed significantly higher concentrations in heparinized compared to citrate plasma. However, levels of adipokines in different plasma samples were highly correlated for most of these markers. We reported that different anticoagulants used in the preparation of the plasma samples affected the measurements of some adipokines. The importance of the present results in epidemiology is relevant when comparing different studies in which blood samples were collected with different anticoagulants. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：43 / 47

页数：5

共 15 条

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