Integrating Oxygen and 3D Cell Culture System: A Simple Tool to Elucidate the Cell Fate Decision of hiPSCs

被引：1

作者：

Khadim, Rubina Rahaman ^{[1
]}

Vadivelu, Raja Kumar ^{[2
,3
]}

Utami, Tia ^{[1
]}

Torizal, Fuad Gandhi ^{[1
]}

Nishikawa, Masaki ^{[2
]}

Sakai, Yasuyuki ^{[1
,2
]}

机构：

[1] Univ Tokyo, Grad Sch Engn, Dept Bioengn, Tokyo 1138654, Japan

[2] Univ Tokyo, Grad Sch Engn, Dept Chem Syst Engn, Tokyo 1138654, Japan

[3] RIKEN Cluster Pioneering Res CPR, RIKEN Hakubi Res Team, Human Biomimet Syst, Wako, Saitama 3510198, Japan

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2022年 / 23卷 / 13期

关键词：

oxygen; permeable; cell fate; early differentiation; EMBRYONIC STEM-CELLS; DIFFERENTIATION; HYPOXIA; MOUSE; EFFICIENT; MEMBRANES; ENDODERM; TISSUES;

D O I：

10.3390/ijms23137272

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oxygen, as an external environmental factor, plays a role in the early differentiation of human stem cells, such as induced pluripotent stem cells (hiPSCs). However, the effect of oxygen concentration on the early-stage differentiation of hiPSC is not fully understood, especially in 3D aggregate cultures. In this study, we cultivated the 3D aggregation of hiPSCs on oxygen-permeable microwells under different oxygen concentrations ranging from 2.5 to 20% and found that the aggregates became larger, corresponding to the increase in oxygen level. In a low oxygen environment, the glycolytic pathway was more profound, and the differentiation markers of the three germ layers were upregulated, suggesting that the oxygen concentration can function as a regulator of differentiation during the early stage of development. In conclusion, culturing stem cells on oxygen-permeable microwells may serve as a platform to investigate the effect of oxygen concentration on diverse cell fate decisions during development.

引用

页数：12

共 47 条

[1] Differentiation of Human Pluripotent Stem Cells Into Definitive Endoderm Cells in Various Flexible Three-Dimensional Cell Culture Systems: Possibilities and Limitations [J].

Bogacheva, Mariia S. ;

Harjumaki, Riina ;

Flander, Emilia ;

Taalas, Ara ;

Bystriakova, Margarita A. ;

Yliperttula, Marjo ;

Xiang, Xiaoqiang ;

Leung, Alan W. ;

Lou, Yan-Ru .

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, 2021, 9

[2] Analysis of SOX2-Expressing Cell Populations Derived from Human Pluripotent Stem Cells [J].

Brafman, David A. ;

Moya, Noel ;

Allen-Soltero, Stephanie ;

Fellner, Thomas ;

Robinson, Megan ;

McMillen, Zoe L. ;

Gaasterland, Terry ;

Willert, Karl .

STEM CELL REPORTS, 2013, 1 (05) :464-478

[3] A Universal System for Highly Efficient Cardiac Differentiation of Human Induced Pluripotent Stem Cells That Eliminates Interline Variability [J].

Burridge, Paul W. ;

Thompson, Susan ;

Millrod, Michal A. ;

Weinberg, Seth ;

Yuan, Xuan ;

Peters, Ann ;

Mahairaki, Vasiliki ;

Koliatsos, Vassilis E. ;

Tung, Leslie ;

Zambidis, Elias T. .

PLOS ONE, 2011, 6 (04)

[4] Engineering in vitro microenvironment for cell bases therapies and drug discovery [J].

Cigognini, Daniela ;

Lomas, Alexander ;

Kumar, Pramod ;

Satyam, Abhigyan ;

English, Andrew ;

Azeem, Ayesha ;

Pandit, Abhay ;

Zeugolis, Dimitrios .

DRUG DISCOVERY TODAY, 2013, 18 (21-22) :1099-1108

[5] The Role of Hypoxia in Development of the Mammalian Embryo [J].

Dunwoodie, Sally L. .

DEVELOPMENTAL CELL, 2009, 17 (06) :755-773

[6] Gas-Permeable Membranes and Co-Culture with Fibroblasts Enable High-density Hepatocyte Culture as Multilayered Liver Tissues [J].

Evenou, Fanny ;

Hamon, Morgan ;

Fujii, Teruo ;

Takeuchi, Shoji ;

Sakai, Yasuyuki .

BIOTECHNOLOGY PROGRESS, 2011, 27 (04) :1146-1153

[7] GATA6 is essential for endoderm formation from human pluripotent stem cells [J].

Fisher, J. B. ;

Pulakanti, K. ;

Rao, S. ;

Duncan, S. A. .

BIOLOGY OPEN, 2017, 6 (07) :1084-1095

[8] The Differential Effects of 2% Oxygen Preconditioning on the Subsequent Differentiation of Mouse and Human Pluripotent Stem Cells [J].

Fynes, Kate ;

Tostoes, Rui ;

Ruban, Ludmila ;

Weil, Ben ;

Mason, Christopher ;

Veraitch, Farlan S. .

STEM CELLS AND DEVELOPMENT, 2014, 23 (16) :1910-1922

[9] Low oxygen tension enhances the generation of lung progenitor cells from mouse embryonic and induced pluripotent stem cells [J].

Garreta, Elena ;

Melo, Esther ;

Navajas, Daniel ;

Farre, Ramon .

PHYSIOLOGICAL REPORTS, 2014, 2 (07)

[10] Methods for advanced hepatocyte cell culture in microwells utilizing air bubbles [J].

Goral, Vasiliy N. ;

Au, Sam H. ;

Faris, Ronald A. ;

Yuen, Po Ki .

LAB ON A CHIP, 2015, 15 (04) :1032-1037

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