Elaboration of a crude antigen ELISA for serodiagnosis of caprine neosporosis:: validation of the test by detection of Neospora caninum-specific antibodies in goats from Sri Lanka

被引：24
作者：
Naguleswaran, A
Hemphill, A
Rajapakse, RPVJ
Sager, H
机构：
[1] Univ Bern, Inst Parasitol, CH-3001 Bern, Switzerland
[2] Univ Peradeniya, Dept Vet Pathobiol, Fac Vet Med & Anim Sci, Peradeniya, Sri Lanka
来源：
VETERINARY PARASITOLOGY | 2004年 / 126卷 / 03期
关键词：
Neospora caninum; goat; serology; ELISA; IFAT; Western blot;
D O I：
10.1016/j.vetpar.2004.08.014
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
The protozoan parasite N. caninum is a major pathogen in cattle and dogs. However, clinical symptoms are occasionally described for other potential hosts. Natural abortion in goats due to N. caninum has been rarely reported and only little data is available on the seroprevalence of N. caninum in this species. In the present study, 486 goats from Sri Lanka were tested in a crude antigen ELISA for the presence of serum antibodies against N. caninum. Additionally, the sera were analysed by N. caninum-Western blot and indirect fluorescence antibody test (IFAT). In all three tests applied, only three sera (0.7%) were scored clearly positive for anti-N. caninum antibodies. The optimal correlation between ELISA, IFAT, and Western blot confirms the suitability of the ELISA for large-scale seroepidemiologic studies, not only in cattle but also in goats. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：257 / 262
页数：6
相关论文
共 14 条
[1]   NEOSPORA-LIKE PROTOZOAL INFECTIONS ASSOCIATED WITH ABORTION IN GOATS [J].
BARR, BC ;
ANDERSON, ML ;
WOODS, LW ;
DUBEY, JP ;
CONRAD, PA .
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 1992, 4 (03) :365-367
[2]   Granulomatous encephalitis in a neurologically impaired goat kid associated with degeneration of Neospora caninum tissue cysts [J].
Corbellini, LG ;
Colodel, EM ;
Driemeier, D .
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2001, 13 (05) :416-419
[3]   Point source exposure of cattle to Neospora caninum consistent with periods of common housing and feeding and related to the introduction of a dog [J].
Dijkstra, T ;
Barkema, HW ;
Heeselink, JW ;
Wouda, W .
VETERINARY PARASITOLOGY, 2002, 105 (02) :89-98
[4]  
Dubey J.P., 2003, J PARASITOL S, V89, pS42
[5]  
Dubey John P., 2003, Korean Journal of Parasitology, V41, P1, DOI 10.3347/kjp.2003.41.1.1
[6]  
Dubey JP, 1996, J AM VET MED ASSOC, V208, P263
[7]   Molecular and immunodiagnostic investigations on bovine neosporosis in Switzerland [J].
Gottstein, B ;
Hentrich, B ;
Wyss, R ;
Thur, B ;
Busato, A ;
Stark, KDC ;
Muller, N .
INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1998, 28 (04) :679-691
[8]   Identification of a major surface protein on Neospora caninum tachyzoites [J].
Hemphill, A ;
Gottstein, B .
PARASITOLOGY RESEARCH, 1996, 82 (06) :497-504
[9]   Evidence suggesting a point source exposure in an outbreak of bovine abortion due to neosporosis [J].
McAllister, MM ;
Huffman, EM ;
Hietala, SK ;
Conrad, PA ;
Anderson, ML ;
Salman, MD .
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 1996, 8 (03) :355-357
[10]   Serological survey and first finding of Neospora caninum in Taiwan, and the detection of its antibodies in various body fluids of cattle [J].
Ooi, HK ;
Huang, CC ;
Yang, CH ;
Lee, SH .
VETERINARY PARASITOLOGY, 2000, 90 (1-2) :47-55
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