Investigation of the interaction of DNA and neutral red by fluorescence spectroscopic analysis

被引：8
|
作者：
Ni, Yong Nian [1 ]
Zhong, Xue Zhi
机构：
[1] Nanchang Univ, Dept Chem, Nanchang 330047, Peoples R China
[2] Nanchang Univ, Key Lab Food Sci MOE, Nanchang 330047, Peoples R China
来源：
CHINESE CHEMICAL LETTERS | 2007年 / 18卷 / 05期
基金：
中国国家自然科学基金;
关键词：
neutral red; DNA; binding constant; fluorescence; chemometrics; singular value decomposition;
D O I：
10.1016/j.cclet.2007.03.027
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The binding characteristics of neutral red (NR) with DNA were investigated by fluorescence spectrometry. Chemometrics approach as singular value decomposition (SVD) was used to evaluate the number of spectral species in the drug-DNA binding process, and then the intrinsic binding constant of 1.6 x 10(4) in base pairs and the binding site number of 0.97 were obtained from the Scatchard plot. (c) 2007 Yong Nian Ni. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.
引用
收藏
页码：569 / 572
页数：4
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