Simultaneous determination of gemcitabine and its metabolite in human plasma by high-performance liquid chromatography

被引：19
作者：
Yilmaz, B
Kadioglu, Y [1 ]
Aksoy, Y
机构：
[1] Ataturk Univ, Fac Pharm, Dept Analyt Chem, TR-25240 Erzurum, Turkey
[2] Ataturk Univ, Fac Med, Dept Urol, TR-25240 Erzurum, Turkey
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2003年 / 791卷 / 1-2期
关键词：
gemcitabine; 2; '-difluoro-2; '-deoxyuridine;
D O I：
10.1016/S1570-0232(03)00211-3
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Gemcitabine (dFdC) is a pyrimidine antimetabolite with broad spectrum activity against tumors. In this paper, a normal-phase high-performance liquid chromatographic method was developed for the determination of the parent drug (dFdC) and its metabolite (dFdU) in human plasma. The described sample preparation procedure for determination of dFdC and dFdU is rapid, sensitive, reproducible and simple. The linear regression equations obtained by least square regression method, were area under the curve=0.0371 concentration (ng ml(-1))+192.53 and 1.05.10(-4) concentration (ng ml(-1))-1.2693 for dFdC and dFdU, respectively. The assay for dFdC and dFdU described in the present report has been applied to plasma samples from a bladder cancer patient. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：103 / 109
页数：7
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