Single 19F Probe for Simultaneous Detection of Multiple Metal Ions Using miCEST MRI

被引：64

作者：

Bar-Shir, Amnon ^{[1
,2
,3
]}

Yadav, Nirbhay N. ^{[1
,7
]}

Gilad, Assaf A. ^{[1
,2
,3
,7
]}

van Zijl, Peter C. M. ^{[1
,7
]}

McMahon, Michael T. ^{[1
,7
]}

Bulte, Jeff W. M. ^{[1
,2
,3
,4
,5
,6
,7
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Russell H Morgan Dept Radiol & Radiol Sci, Baltimore, MD 21205 USA

[2] Johns Hopkins Univ, Sch Med, Inst Cell Engn, Cellular Imaging Sect, Baltimore, MD 21205 USA

[3] Johns Hopkins Univ, Sch Med, Inst Cell Engn, Vasc Biol Program, Baltimore, MD 21205 USA

[4] Johns Hopkins Univ, Sch Med, Dept Biomed Engn, Baltimore, MD 21205 USA

[5] Johns Hopkins Univ, Sch Med, Dept Chem & Biomol Engn, Baltimore, MD 21205 USA

[6] Johns Hopkins Univ, Sch Med, Dept Oncol, Baltimore, MD 21205 USA

[7] Kennedy Krieger Inst, FM Kirby Res Ctr Funct Brain Imaging, Baltimore, MD 21205 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2015年 / 137卷 / 01期

关键词：

IMAGING IN-VIVO; MAGNETIC-RESONANCE; CONTRAST AGENT; NONINVASIVE MRI; CALCIUM; NANOPARTICLES; NMR; INDICATORS; COMPLEXES; CEST;

D O I：

10.1021/ja511313k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The local presence and concentration of metal ions in biological systems has been extensively studied ex vivo using fluorescent dyes. However, the detection of multiple metal ions in vivo remains a major challenge. We present a magnetic resonance imaging (MRI)-based method for noninvasive detection of specific ions that may be coexisting, using the tetrafluorinated derivative of the BAPTA (TF-BAPTA) chelate as a F-19 chelate analogue of existing optical dyes. Taking advantage of the difference in the ion-specific F-19 nuclear magnetic resonance (NMR) chemical shift offset (Delta omega) values between the ion-bound and free TF-BAPTA, we exploited the dynamic exchange between ion-bound and free TF-BAPTA to obtain MRI contrast with multi-ion chemical exchange saturation transfer (miCEST). We demonstrate that TF-BAPTA as a prototype single 19F probe can be used to separately visualize mixed Zn2+ and Fe2+ ions in a specific and simultaneous fashion, without interference from potential competitive ions.

引用

页码：78 / 81

页数：4

共 39 条

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