BoCER1 is essential for the synthesis of cuticular wax in cabbage (Brassica oleracea L. var. capitata)

被引：16

作者：

Cao, Wenxue ^{[1
]}

Dong, Xin ^{[1
]}

Ji, Jialei ^{[1
]}

Yang, Limei ^{[1
]}

Fang, Zhiyuan ^{[1
]}

Zhuang, Mu ^{[1
]}

Zhang, Yangyong ^{[1
]}

Lv, Honghao ^{[1
]}

Wang, Yong ^{[1
]}

Sun, Peitian ^{[1
]}

Liu, Yumei ^{[1
]}

Li, Zhansheng ^{[1
]}

Han, Fengqing ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Vegetables & Flowers, Key Lab Biol & Genet Improvement Hort Crops, Minist Agr, Beijing 100081, Peoples R China

来源：

SCIENTIA HORTICULTURAE | 2021年 / 277卷

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

Cabbage; BoCER1; CRISPR/Cas9; Gene function; Molecular breeding; DROUGHT TOLERANCE; PLANT; BIOSYNTHESIS; GENE; EXPRESSION; TOMATO;

D O I：

10.1016/j.scienta.2020.109801

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

Leafy head color is a very important trait in cabbage breeding. In previous studies, BoCER1 was identified as a candidate gene that was found to be closely related to the glossy phenotype in cabbage lines 10Q-961 and g21-3. In this study, we found that the BoCER1 was expressed in all cabbage tissues, except roots. Moreover, to determine the function of BoCER1 in cabbage, the gene editing vector CRISPR-BoCER1 was constructed and transformed into the wildtype cabbage 'CW1'. Three plants with edited genomes were obtained: CW1-1, CW1-2 and CW1-3. Scanning electron microscopy and gas chromatography mass spectrometry analyses of CW1-3 showed that the wax content was significantly reduced compared with the wildtype. The results showed that BoCER1 plays an important role in the biosynthesis of cuticular wax in cabbage. Moreover, the wax-less material created by CRISPR/Cas9 could be used for the breeding of brilliant green cabbage.

引用

页数：6

共 50 条

[31] Variability of characteristics in new experimental hybrids of early cabbage (Brassica oleracea var. capitata L.)
Janko, Cervenski
Jelica, Gvozdanovic-Varga
Svetlana, Glogovac
Sasa, Dragin
AFRICAN JOURNAL OF BIOTECHNOLOGY, 2011, 10 (59): : 12555 - 12560
[32] Study on transformation of cysteine proteinase inhibitor gene into cabbage (Brassica oleracea var. capitata L.)
Lei, Jian Jun
Yang, Wen Jie
Yuan, Song Hong
Ying, Fu You
Qiong, Li Cheng
PROCEEDINGS OF THE JOINT MEETING OF THE FOURTEENTH CRUCIFER GENETICS WORKSHOP AND FOURTH ISHS SYMPOSIUM ON BRASSICAS, 2006, (706): : 231 - +
[33] Effect of genotype/year interaction on yield components in cabbage -: (Brassica oleracea var. capitata L.)
Cervenski, J
Gvozdenovic, D
Gvozdanovic-Varga, J
Vasic, M
Takac, A
PROCEEDINGS OF THE SECOND BALKAN SYMPOSIUM ON VEGETABLES AND POTATOES, 2002, (579): : 57 - 58
[34] Preliminary Study of the Characteristics of Several Glossy Cabbage(Brassica oleracea var. capitata L.) Mutants
TANG Jun
LIU Dongming
LIU Zezhou
YANG Limei
FANG Zhiyuan
LIU Yumei
ZHUANG Mu
ZHANG Yangyong
Lü Honghao
YI Dengxia
SUN Peitian
Horticultural Plant Journal, 2015, 1 (02) : 93 - 100
[35] Interdependence of Traits in Early-Maturing Genotypes of Cabbage (Brassica oleracea var. capitata L.)
Cervenski, J.
Gvozdenovic, D.
Gvozdanovic-Varga, J.
Bugarski, D.
Vasic, M.
Glogovac, S.
V BALKAN SYMPOSIUM ON VEGETABLES AND POTATOES, 2012, 960 : 99 - 104
[36] Breeding materials for resistance to Verticillium wilt in Japanese cabbage (Brassica oleracea L. var. capitata)
Kemmochi, I
Kobayashi, I
Tsuchiya, M
Sakai, H
Shimizu, M
JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE, 2000, 69 (04): : 483 - 491
[37] Local cabbage (Brassica oleracea var. capitata L.) populations from Serbian Province of Vojvodina
Janko, Cervenski
Jelica, Gvozdanovic-Varga
Svetlana, Glogovac
AFRICAN JOURNAL OF BIOTECHNOLOGY, 2011, 10 (27): : 5281 - 5285
[38] High Frequency Organogenesis in Cotyledon and Hypocotyl Explants of Cabbage (Brassica oleracea L. var. capitata)
Shalini Sharma
Geetika Gambhir
D. K. Srivastava
National Academy Science Letters, 2014, 37 : 5 - 12
[39] Pesticide residues in the spring cabbage (Brassica oleracea L. var. capitata) grown in open field
Zhang, Zhi-Yong
Liu, Xian-Jin
Yu, Xiang-Yang
Zhang, Cun-Zheng
Hong, Xiao-Yue
FOOD CONTROL, 2007, 18 (06) : 723 - 730
[40] Improvements of doubled haploid production protocol for white cabbage (Brassica oleracea var. capitata L.)
Pilih, Katarina Rudolf
Potokar, Urska Karolina
Bohanec, Borut
FOLIA HORTICULTURAE, 2018, 30 (01) : 57 - 66

← 1 2 3 4 5 →