Characterization of flavonoid subgroups and hydroxy substitution by HPLC-MS/MS

被引:249
作者
Tsimogiannis, Dimitrios
Samiotaki, Martina
Panayotou, George
Oreopoulou, Vassiliki
机构
[1] Natl Tech Univ Athens, Sch Chem Engn, Athens 15780, Greece
[2] Biomed Sci Res Ctr Alexander Fleming, Vari 16672, Greece
关键词
HPLC-MS/MS analysis; flavonoids; Origanum vulgare;
D O I
10.3390/12030593
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
HPLC-DAD coupled with mass spectrometry in the positive ionization mode was applied to study the fragmentation of twelve selected flavonoids. Compounds belonging to all the major subgroups found in common plants, i.e. flavonols, flavones, dihydroflavonols, flavanones and flavanols were studied. Compound standards were injected into the spectrometer and produced characteristic mass spectra. The fragmentation of each compound was studied and it was shown that the dehydration and carbon monoxide losses from the [M+H](+) ion by the members of each subgroup produced specific fragments, thus allowing the characterization of the flavonoid subgroups. Moreover, fragments resulting from fission of the C-rings are specific of each subgroup and revealed the substitution pattern of A- and B-rings. In order to verify the identifying efficiency of the positive ionization mode through these characteristic fragmentations, the unknown flavonoids of an Origanum vulgare diethyl ether extract were separated with the HPLC system and the major peaks were successfully identified with the mass spectrometer.
引用
收藏
页码:593 / 606
页数:14
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