Serum Metabolomic Characterization of Liver Fibrosis in Rats and Anti-Fibrotic Effects of Yin-Chen-Hao-Tang

被引：33

作者：

Zhang, Hongyang ^{[1
]}

Wang, Xiaoning ^{[2
]}

Hu, Ping ^{[1
]}

Zhou, Wenjun ^{[2
]}

Zhang, Min ^{[3
]}

Liu, Jia ^{[2
]}

Wang, Yuerong ^{[1
]}

Liu, Ping ^{[2
,4
]}

Luo, Guoan ^{[3
,5
]}

机构：

[1] E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

[2] Shanghai Univ Tradit Chinese Med, E Inst Shanghai Municipal Educ Comm, Shanghai 201203, Peoples R China

[3] E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China

[4] Shuguang Hosp, Inst Liver Dis, Minist Educ, Key Lab Liver & Kidney Dis, Shanghai 201203, Peoples R China

[5] Tsinghua Univ, Dept Chem, Beijing 100084, Peoples R China

来源：

MOLECULES | 2016年 / 21卷 / 01期

基金：

中国国家自然科学基金; 上海市自然科学基金;

关键词：

serum metabolomics; liver fibrosis; Yin-Chen-Hao-Tang; anti-fibrotic effects; oxidative stress; lipid peroxidation; ultra-performance liquid chromatography-time-of-flight mass spectrometry; partial least squares-discriminant analysis; HEPATIC STELLATE CELLS; MULTIVARIATE-ANALYSIS; MASS-SPECTROMETRY; CHLOROGENIC ACID; GENE-EXPRESSION; DECOCTION; CONSTITUENTS; METABONOMICS; INFLAMMATION; ANTIOXIDANT;

D O I：

10.3390/molecules21010126

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Yin-Chen-Hao-Tang (YCHT) is a famous Chinese medicine formula which has long been used in clinical practice for treating various liver diseases, such as liver fibrosis. However, to date, the mechanism for its anti-fibrotic effects remains unclear. In this paper, an ultra-performance liquid chromatography-time-of-flight mass spectrometry (UPLC-TOF-MS)-based metabolomic study was performed to characterize dimethylnitrosamine (DMN)-induced liver fibrosis in rats and evaluate the therapeutic effects of YCHT. Partial least squares-discriminant analysis (PLS-DA) showed that the model group was well separated from the control group, whereas the YCHT-treated group exhibited a tendency to restore to the controls. Seven significantly changed fibrosis-related metabolites, including unsaturated fatty acids and lysophosphatidylcholines (Lyso-PCs), were identified. Moreover, statistical analysis demonstrated that YCHT treatment could reverse the levels of most metabolites close to the normal levels. These results, along with histological and biochemical examinations, indicate that YCHT has anti-fibrotic effects, which may be due to the suppression of oxidative stress and resulting lipid peroxidation involved in hepatic fibrogenesis. This study offers new opportunities to improve our understanding of liver fibrosis and the anti-fibrotic mechanisms of YCHT.

引用

页数：12

共 43 条

[41] Development of a Robust and Repeatable UPLC-MS Method for the Long-Term Metabolomic Study of Human Serum [J].

Zelena, Eva ;

Dunn, Warwick B. ;

Broadhurst, David ;

Francis-McIntyre, Sue ;

Carroll, Kathleen M. ;

Begley, Paul ;

O'Hagan, Steve ;

Knowles, Joshua D. ;

Halsall, Antony ;

Wilson, Ian D. ;

Kellt, Douglas B. .

ANALYTICAL CHEMISTRY, 2009, 81 (04) :1357-1364

[42] Metabolomic profiling of rat serum associated with isoproterenol-induced myocardial infarction using ultra-performance liquid chromatography/time-of-flight mass spectrometry and multivariate analysis [J].

Zhang, Hong-yang ;

Chen, Xi ;

Hu, Ping ;

Liang, Qiong-lin ;

Liang, Xiao-ping ;

Wang, Yi-ming ;

Luo, Guo-an .

TALANTA, 2009, 79 (02) :254-259

[43] A cellular lipidomic study on the Aβ-induced neurotoxicity and neuroprotective effects of EGCG by using UPLC/MS-based glycerolipids profiling and multivariate analysis [J].

Zhang, Hongyang ;

Wang, Jing-Rong ;

Yau, Lee Fong ;

Ho, Hing Man ;

Chan, Chi Leung ;

Hu, Ping ;

Liu, Liang ;

Jiang, Zhi-Hong .

MOLECULAR BIOSYSTEMS, 2012, 8 (12) :3208-3215

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