Purification and primary structure of two isoforms of arenicin, a novel antimicrobial peptide from marine polychaeta Arenicola marina

被引：126

作者：

Ovchinnikova, TV

Aleshina, GM

Balandin, SV

Krasnosdembskaya, AD

Markelov, ML

Frolova, EI

Leonova, YF

Tagaev, AA

Krasnodembsky, EG

Kokryakov, VN

机构：

[1] Russian Acad Sci, Shemyakin & Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia

[2] Russian Acad Med Sci, Inst Expt Med, St Petersburg 197376, Russia

[3] St Petersburg State Univ, St Petersburg 199034, Russia

来源：

FEBS LETTERS | 2004年 / 577卷 / 1-2期

关键词：

antimicrobial peptide; arenicin; lugworm; marine invertebrate; innate immunity; primary structure; cDNA; precursor; Arenicola marina;

D O I：

10.1016/j.febslet.2004.10.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two novel 21-residue antimicrobial peptides, arenicin-1 and arenicin-2, exhibiting activity against Gram-positive and Gram-negative bacteria and fungi, were purified from coelomocytes of marine polychaeta Arenicola marina (lugworm) by preparative gel electrophoresis and RP-HPLC. Molecular masses (2758.3 and 2772.3 Da) and complete amino acid sequences (RWCVYAYVRVRGVLVRYRRCW and RWCVYAYVRIRGVLVRYRRCW)(1) were determined for each isoform. Each arenicin has one disulfide bond (Cys3-Cys20). The total RNA was isolated from the lugworm coelomocytes, RT-PCR and cloning were performed, and cDNA was sequenced. A 202-residue preproarenicin contains a putative signal peptide (25 amino acids) and a long prodomain. Arenicins have no structure similarity to any previously identified antimicrobial peptides. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：209 / 214

页数：6

共 41 条

[1] Multiple antimicrobial peptides and peptides related to bradykinin and neuromedin N isolated from skin secretions of the pickerel frog, Rana palustris [J].