Melatonin pretreatment modulates anti-inflammatory, antioxidant, YKL-40, and matrix metalloproteinases in endotoxemic rat lung tissue

被引:10
作者
Ates, Gulten [1 ]
Tamer, Sule [2 ]
Yorulmaz, Hatice [3 ]
Mutlu, Sevcihan [4 ]
Olgac, Vakur [5 ]
Aksu, Abdullah [6 ]
Caglar, Nuray Balkis [6 ]
Ozkok, Elif [7 ]
机构
[1] Istanbul Yeni Yuzyil Univ, Fac Med, Dept Physiol, TR-34010 Istanbul, Turkey
[2] Istanbul Univ, Istanbul Med Fac, Dept Physiol, TR-34093 Istanbul, Turkey
[3] Halic Univ, Fac Hlth Sci, TR-34445 Istanbul, Turkey
[4] Istanbul Univ, Inst Oncol, Dept Clin Oncol, TR-34093 Istanbul, Turkey
[5] Istanbul Univ, Inst Oncol, Dept Pathol, TR-34093 Istanbul, Turkey
[6] Istanbul Univ, Inst Marine Sci & Management, Dept Chem Oceanog, TR-34134 Istanbul, Turkey
[7] Istanbul Univ, Aziz Sancar Inst Expt Med, Dept Neurosci, TR-34093 Istanbul, Turkey
关键词
Melatonin; YKL-40; matrix metalloproteinases; oxidative stress; cytokines; lipopolysaccharide; ORGAN DYSFUNCTION SYNDROME; OXIDATIVE STRESS; MITOCHONDRIAL-FUNCTION; SEPTIC SHOCK; MOUSE MODEL; EXPRESSION; SEPSIS; ACTIVATION; INJURY; LIVER;
D O I
10.1177/15353702221084933
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
We aimed to investigate the effects of melatonin administered before and during endotoxemia on the lung tissue of rats, cytokine, YKL-40, matrix metalloproteinase (MMP) and inhibitor levels, oxidative stress parameters, and energy balance. Sepsis was induced with lipopolysaccharide (LPS), the cell wall molecule of gram negative bacteria. Rats were divided into four groups, Control, LPS (Escherichia coli O127:B8, 20 mg/kg), melatonin (10 mg/kg), and melatonin+LPS (M+LPS). After injections, lung tissues samples were taken for experimental analyses. YKL-40, thiobarbituric acid reactive substances (TBARS), glutathione reductase (GR), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) enzymes levels were measured, high-energy components were analyzed; tumor necrosis factor-alpha (TNF-alpha), MMP-2, YKL-40, MMP-9, myeloperoxidase (MPO), tissue inhibitors of matrix metalloproteinase (TIMP)-1, and interleukin (IL)-10 immunoreactivities were investigated. In LPS group, YKL-40, creatine phosphate (both, p < 0.05), SOD, GR, adenosine mono-phophate (AMP), adenosine tri-phosphate (ATP) (for all, p < 0.01) were significantly decreased, while TBARS and adenosine di-phosphate (ADP) levels were increased (p < 0.01, p < 0.05; respectively) compared to other groups. MMP-2 and -9, TIMP-1, TNF-alpha, IL-10, and MPO immunoreactivity were investigated in LPS group. On the contrary, in M+LPS group, MMP-9, TIMP-1 immunoreactivities were not found and IL-10 and MMP-2 immunoreactivities were found with little involvement. In M+LPS group, YKL-40, GR, AMP, ATP, creatine phosphate (for all, p < 0.05), and SOD (p < 0.01) levels were significantly increased and TBARS levels were decreased (p < 0.05). In our study, we suggest that melatonin exerts a protective and curative effect by reducing the matrix metalloproteinase levels responsible for tissue damage balance, stimulating the release of antioxidant enzymes, regulating cytokines and energy balance during endotoxemia.
引用
收藏
页码:1080 / 1089
页数:10
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