An evolutionarily diverged mitochondrial protein controls biofilm growth and virulence in Candida albicans

被引:10
|
作者
Mamouei, Zeinab [1 ]
Singh, Shakti [2 ]
Lemire, Bernard [3 ]
Gu, Yiyou [2 ]
Alqarihi, Abdullah [2 ]
Nabeela, Sunna [2 ]
Li, Dongmei [4 ]
Ibrahim, Ashraf [1 ,2 ]
Uppuluri, Priya [1 ,2 ]
机构
[1] Univ Calif Los Angeles, David Geffen Sch Med, Los Angeles, CA 90095 USA
[2] Harbor UCLA Med Ctr, Lundquist Inst Biomed Innovat, Div Infect Dis, Torrance, CA 90509 USA
[3] Univ Alberta, Dept Biochem, Edmonton, AB, Canada
[4] Georgetown Univ, Med Ctr, Dept Microbiol & Immunol, Washington, DC 20007 USA
关键词
D O I
10.1371/journal.pbio.3000957
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A forward genetic screening approach identified orf19.2500 as a gene controlling Candida albicans biofilm dispersal and biofilm detachment. Three-dimensional (3D) protein modeling and bioinformatics revealed that orf19.2500 is a conserved mitochondrial protein, structurally similar to, but functionally diverged from, the squalene/phytoene synthases family. The C. albicans orf19.2500 is distinguished by 3 evolutionarily acquired stretches of amino acid inserts, absent from all other eukaryotes except a small number of ascomycete fungi. Biochemical assays showed that orf19.2500 is required for the assembly and activity of the NADH ubiquinone oxidoreductase Complex I (CI) of the respiratory electron transport chain (ETC) and was thereby named NDU1. NDU1 is essential for respiration and growth on alternative carbon sources, important for immune evasion, required for virulence in a mouse model of hematogenously disseminated candidiasis, and for potentiating resistance to antifungal drugs. Our study is the first report on a protein that sets the Candida-like fungi phylogenetically apart from all other eukaryotes, based solely on evolutionary "gain" of new amino acid inserts that are also the functional hub of the protein.
引用
收藏
页数:27
相关论文
共 50 条
  • [31] Effect of Jujube Honey on Candida albicans Growth and Biofilm Formation
    Ansari, Mohammad Javed
    Al-Ghamdi, Ahmad
    Usmani, Salma
    Al-Waili, Noori S.
    Sharma, Deepak
    Nuru, Adgaba
    Al-Attal, Yehya
    ARCHIVES OF MEDICAL RESEARCH, 2013, 44 (05) : 352 - 360
  • [32] Zingerone effect against Candida albicans growth and biofilm production
    Chougule, Sayali
    Basrani, Sargun
    Gavandi, Tanjila
    Patil, Shivani
    Yankanchi, Shivanand
    Jadhav, Ashwini
    Karuppayil, Sankunny Mohan
    JOURNAL DE MYCOLOGIE MEDICALE, 2025, 35 (01):
  • [33] Evaluation of the Influence of Antifungal Drugs on Growth of Candida albicans in the Biofilm
    Lisovskaya S.
    Khaldeeva E.
    Salmakova A.
    Kulikov S.
    BioNanoScience, 2016, 6 (4) : 588 - 590
  • [34] The Candida albicans EED1 gene encodes a key protein for filamentous growth and virulence
    Martin, R.
    Jacobsen, I.
    Albrecht, A.
    Hube, B.
    MYCOSES, 2008, 51 (05) : 415 - 415
  • [35] Rfg1, a protein related to the Saccharomyces cerevisiae hypoxic regulator Rox1, controls filamentous growth and virulence in Candida albicans
    Kadosh, D
    Johnson, AD
    MOLECULAR AND CELLULAR BIOLOGY, 2001, 21 (07) : 2496 - 2505
  • [36] How does temperature trigger biofilm adhesion and growth in Candida albicans and two non-Candida albicans Candida species?
    Casagrande Pierantoni, Debora
    Corte, Laura
    Casadevall, Arturo
    Robert, Vincent
    Cardinali, Gianluigi
    Tascini, Carlo
    MYCOSES, 2021, 64 (11) : 1412 - 1421
  • [37] Filamentous Non-albicans Candida Species Adhere to Candida albicans and Benefit From Dual Biofilm Growth
    Pathirana, Ruvini U.
    McCall, Andrew D.
    Norris, Hannah L.
    Edgerton, Mira
    FRONTIERS IN MICROBIOLOGY, 2019, 10
  • [38] Temporal Profile of Biofilm Formation, Gene Expression and Virulence Analysis in Candida albicans Strains
    de Barros, Patricia Pimentel
    Rossoni, Rodnei Dennis
    Ribeiro, Felipe De Camargo
    Junqueira, Juliana Campos
    Cardoso Jorge, Antonio Olavo
    MYCOPATHOLOGIA, 2017, 182 (3-4) : 285 - 295
  • [39] Novel role of a family of major facilitator transporters in biofilm development and virulence of Candida albicans
    Shah, Abdul Haseeb
    Singh, Ashutosh
    Dhamgaye, Sanjiveeni
    Chauhan, Neeraj
    Vandeputte, Patrick
    Suneetha, Korivi Jyothiraj
    Kaur, Rupinder
    Mukherjee, Pranab K.
    Chandra, Jyotsna
    Ghannoum, Mahmoud A.
    Sanglard, Dominique
    Goswami, Shyamal K.
    Prasad, Rajendra
    BIOCHEMICAL JOURNAL, 2014, 460 : 223 - 235
  • [40] Temporal Profile of Biofilm Formation, Gene Expression and Virulence Analysis in Candida albicans Strains
    Patrícia Pimentel de Barros
    Rodnei Dennis Rossoni
    Felipe De Camargo Ribeiro
    Juliana Campos Junqueira
    Antonio Olavo Cardoso Jorge
    Mycopathologia, 2017, 182 : 285 - 295