Proteomic tools for cell biology

被引：33

作者：

McDonald, WH ^{[1
]}

Yates, JR ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA

来源：

TRAFFIC | 2000年 / 1卷 / 10期

关键词：

cell biology; proteome; proteomics; mass spectrometry; two-dimensional gel electrophoresis;

D O I：

10.1034/j.1600-0854.2000.011001.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Acquisition of large bodies of genomic sequence is facilitating the use of global techniques to assay cellular function. DNA microarrays have enabled the measurement of global mRNA levels and are able to detect changes in gene expression between different cellular states. Since much of the regulation of physiolgical processes happens post-translationally. measuring only the mRNA levels gives an incomplete picture. Strategies to assay global expression, localization, or interaction of proteins fall into the emerging field of proteomics, with various combinations of techniques being utilized to separate and identify proteins. In this review, we will present a general overview of the currently available proteomic tools and then give examples of how these tools are being utilized to answer questions in cell biology.

引用

页码：747 / 754

页数：8

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