The influence of nano-scale surface roughness on bacterial adhesion to ultrafine-grained titanium

被引：366

作者：

Truong, Vi K. ^{[1
]}

Lapovok, Rimma ^{[2
,3
]}

Estrin, Yuri S. ^{[2
,3
,4
]}

Rundell, Stuart ^{[2
]}

Wang, James Y. ^{[5
]}

Fluke, Christopher J. ^{[6
]}

Crawford, Russell J. ^{[1
]}

Ivanova, Elena R. ^{[1
]}

机构：

[1] Swinburne Univ Technol, Fac Life & Social Sci, Hawthorn, Vic 3122, Australia

[2] Monash Univ, Dept Mat Engn, Clayton, Vic 3800, Australia

[3] ARC Ctr Excellence Design Light Met, Clayton, Vic 3800, Australia

[4] CSIRO, Div Proc Sci & Engn, Clayton, Vic 3168, Australia

[5] Swinburne Univ Technol, IRIS, Hawthorn, Vic 3122, Australia

[6] Swinburne Univ Technol, Ctr Astrophys & Supercomp, Hawthorn, Vic 3122, Australia

来源：

BIOMATERIALS | 2010年 / 31卷 / 13期

基金：

澳大利亚研究理事会; 新加坡国家研究基金会;

关键词：

Titanium surfaces; Equal channel angular pressing (ECAP); Bacterial adhesion; Staphylococcus aureus; Pseudomonas aeruginosa; Bioimplant materials; SEVERE PLASTIC-DEFORMATION; BIOMEDICAL APPLICATIONS; PSEUDOMONAS-AERUGINOSA; OSTEOBLAST ADHESION; NANOSCALE ROUGHNESS; NANOPHASE CERAMICS; ENHANCED FUNCTIONS; ESCHERICHIA-COLI; CELL-ADHESION; TOPOGRAPHY;

D O I：

10.1016/j.biomaterials.2010.01.071

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

We discuss the effect of extreme grain refinement in the bulk of commercial purity titanium (CP, Grade-2) on bacterial attachment to the mechano-chemically polished surfaces of the material. The ultrafine crystallinity of the bulk was achieved by severe plastic deformation by means of equal channel angular pressing (ECAP). The chemical composition, wettability, surface topography and roughness of titanium surfaces were characterized using X-ray photoelectron spectroscopy (XPS) and water contact angle (WCA) measurements, as well as atomic force microscopy (AFM) with 3D interactive visualization of the titanium surface morphology. It was found that physico-chemical surface characteristics of the as-received and the ECAP-modified CP titanium did not differ in any significant way, while the surface roughness at the nano-scale did. Optical profilometry performed on large scanning areas of approximately 225 mu m x 300 mu m showed that there was no significant difference between the roughness parameters R-a and R-q for surfaces in the two conditions, the overall level of roughness being lower for the ECAP-processed one. By contrast, topographic profile analysis at the nano-scale by AFM did reveal a difference in these parameters. This difference was sensitive to the size of the scanned surface area. A further two surface roughness parameters, skewness (R-skw) and kurtosis (R-kur), were also used to describe the morphology of titanium surfaces. It was found that the bacterial strains used in this study as adsorbates, viz. Staphylococcus aureus CIP 65.8 and Pseudomonas aeruginosa ATCC 9025, showed preference for surfaces of ECAP-processed titanium. S. aureus cells were found to have a greater propensity for attachment to surfaces of ECAP-modified titanium, while the attachment of P aeruginosa, while also showing some preference for the ECAP-processed material, was less sensitive to the ECAP processing. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：3674 / 3683

页数：10

共 48 条

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