A rapid, cost-effective procedure for the extraction of microbial DNA from soil

被引：20
|
作者：
Wechter, P [1 ]
Williamson, J [1 ]
Robertson, A [1 ]
Kluepfel, D [1 ]
机构：
[1] Clemson Univ, Dept Plant Pathol & Physiol, Clemson, SC 29634 USA
来源：
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2003年 / 19卷 / 01期
关键词：
purification of soil DNA; soil DNA; soil DNA extraction; soil microbiology;
D O I：
10.1023/A:1022587806945
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Here we describe a DNA extraction method that is based on a simple, rapid polyvinylpolypyrrolidone-calcium chloride precipitation to release microorganisms from the soil combined with lysozyme proteinase-SDS lysis of the microbial community. The extracted DNA is of high quality and allows direct detection of specific genes by the polymerase chain reaction (PCR) as well as cloning of indigenous microbial DNA. This method facilitates the extraction of 36 500-mg soil samples simultaneously in a 2-h period by one person. The procedure is safe, inexpensive, and does not require specialized equipment or generate hazardous wastes.
引用
收藏
页码：85 / 91
页数：7
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