In vivo validation of free-space fluorescence tomography using nuclear imaging

被引：17

作者：

Garofalakis, Anikitos ^{[1
,2
]}

Dubois, Albertine ^{[1
,2
]}

Kuhnast, Bertrand ^{[1
]}

Dupont, Daniel M. ^{[1
,2
]}

Janssens, Isabelle ^{[1
,2
]}

Mackiewicz, Nicolas ^{[1
,2
]}

Dolle, Frederic ^{[1
]}

Tavitian, Bertrand ^{[1
,2
]}

Duconge, Frederic ^{[1
,2
]}

机构：

[1] Commissariat Energie Atom & Energies Alternat, I2BM, SHFJ, F-91401 Orsay, France

[2] INSERM, Lab Imagerie Mol Expt, U1023, F-91401 Orsay, France

来源：

OPTICS LETTERS | 2010年 / 35卷 / 18期

关键词：

MOLECULAR TOMOGRAPHY;

D O I：

10.1364/OL.35.003024

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

The performance of small animal photonic imaging has been considerably improved since the development of fluorescence diffuse optical tomography (fDOT), which can reconstruct fluorescent probe distribution inside tissue. However, the quantification capabilities of this new technology are still a topic of debate, especially in comparison to classical nuclear imaging techniques. Here, we present a method to in vivo calibrate the quantity and localization of a probe provided by free-space fDOT (where no plate is compressing the mouse) with positron emission tomography (PET) and x-ray computed tomography, respectively. This methodology allowed us to demonstrate a strong linear correlation (R-2 = 0.95) between fDOT and PET for probe concentrations ranging from 3 nM to 1 mu M in a deep-seated organ. (C) 2010 Optical Society of America

引用

页码：3024 / 3026

页数：3

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