Increased susceptibility of cholangiocytes to tumor necrosis factor-α cytotoxicity after bile duct ligation

被引：20

作者：

Alpini, G

Ueno, Y

Tadlock, L

Glaser, SS

LeSage, G

Francis, H

Taffetani, S

Marzioni, M

Alvaro, D

Patel, T

机构：

[1] Texas A&M Univ Syst, Hlth Sci Ctr, Coll Med, Temple, TX 76504 USA

[2] Scott & White Mem Hosp & Clin, Dept Internal Med, Temple, TX 76504 USA

[3] Scott & White Mem Hosp & Clin, Dept Med Phys, Temple, TX 76504 USA

[4] Scott & White Mem Hosp & Clin, Div Res & Educ, Temple, TX 76504 USA

[5] Cent Texas Vet Hlth Care Syst, Temple, TX 76504 USA

[6] Tohoku Univ, Sch Med, Div Gastroenterol, Sendai, Miyagi 9808574, Japan

[7] Univ Roma La Sapienza, Div Gastroenterol, I-00185 Rome, Italy

来源：

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 2003年 / 285卷 / 01期

关键词：

apoptosis; bile flow; intrahepatic biliary epithelium; proliferation; secretin;

D O I：

10.1152/ajpcell.00497.2002

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Tumor necrosis factor (TNF)-alpha plays a critical role in epithelial cell injury. However, the role of TNF-alpha in mediating cholangiocyte injury under physiological or pathophysiological conditions is unknown. Thus we assessed the effects of TNF-alpha alone or following sensitization by actinomycin D on cell apoptosis, proliferation, and basal and secretin-stimulated ductal secretion in cholangiocytes from normal or bile duct-ligated (BDL) rats. Cholangiocytes from normal or BDL rats were highly resistant to TNF-alpha alone. However, presensitization by actinomycin D increased apoptosis in cholangiocytes following BDL and was associated with an inhibition of proliferation and secretin-stimulated ductal secretion. Thus TNF-alpha mediates cholangiocyte injury and altered ductal secretion following bile duct ligation. These observations suggest that cholestasis may enhance susceptibility to cytokine-mediated cholangiocyte injury.

引用

页码：C183 / C194

页数：12