Sperm IZUMO1-Dependent Gamete Fusion Influences Male Fertility in Mice

被引：8
作者：
Saito, Takako [1 ]
Wada, Ikuo [1 ]
Inoue, Naokazu [1 ]
机构：
[1] Fukushima Med Univ, Sch Med, Inst Biomed Sci, Dept Cell Sci, 1 Hikarigaoka, Fukushima, Fukushima 9601295, Japan
来源：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2019年 / 20卷 / 19期
关键词：
spermatozoon; IZUMO1; biomarker; male fertility; ARTIFICIAL-INSEMINATION; PROTEIN IZUMO; IN-VITRO; EGG; JUNO; CONTRACEPTION; RECEPTOR; CD9;
D O I：
10.3390/ijms20194809
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Sperm-egg fusion is accomplished through the interaction of a specific set of membrane proteins in each gamete: sperm IZUMO1 and oocyte JUNO. Recently, we found that alternative splicing of the Izumo1 gene generates a novel IZUMO1 isoform (IZUMO1_v2). Here, we obtained four mouse lines, having graded different levels of IZUMO1 protein by combining an original IZUMO1 (IZUMO1_v1) knockout with IZUMO1-null (both IZUMO1_v1 and _v2 disrupted) genetic background, in order to determine how the quantity of IZUMO1 influences male fertility. Subsequently, we clarified that the signal intensity from two quantitative assays, western blot and immunostaining analyses with a monoclonal antibody against mouse IZUMO1, were strongly correlated with average litter size. These results suggest that evaluating IZUMO1 protein levels is useful for predicting fecundity, and is a suitable test for male fertility.
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页数：9
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