Recent advances in the molecular diagnosis of mucormycosis

被引:57
作者
Dadwal, Sanjeet S. [1 ]
Kontoyiannis, Dimitrios P. [2 ]
机构
[1] City Hope Natl Med Ctr, Div Infect Dis, 1500 E Duarte Rd, Duarte, CA 91010 USA
[2] Univ Texas MD Anderson Canc Ctr, Dept Infect Dis Infect Control & Employee Hlth, Houston, TX 77030 USA
关键词
Molecular diagnosis; mucormycosis; biomarkers; early diagnosis; mycology; FLIGHT MASS-SPECTROMETRY; DESORPTION IONIZATION-TIME; BRONCHOALVEOLAR LAVAGE SAMPLES; MALDI-TOF MS; PCR ASSAY; IMMUNOCOMPROMISED PATIENTS; HEMATOLOGIC MALIGNANCIES; CEREBRAL MUCORMYCOSIS; RHIZOPUS-MICROSPORUS; CEREBROSPINAL-FLUID;
D O I
10.1080/14737159.2018.1522250
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Introduction: Fungal infection burden related to Mucorales has been on the rise with significant associated morbidity and mortality. The major obstacle in the management has been lack of a non-invasive rapid and a reliable diagnostic test. Developing a culture-independent biomarker for the early diagnosis of mucormycosis is a major unmet need in modern mycology. Several approaches have been developed, such as immunohistochemistry (IHC) that can confirm the histopathologic diagnosis of the invasive mold infection, polymerase chain reaction (PCR) on formalin-fixed paraffin-embedded (FFPE) or fresh tissue, body fluids such as bronchoalveolar fluid (BAL), and detection directly from serum/blood. Serologic tests, matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS), metabolomics and metagenomic shotgun sequencing are other evolving technologies. Area covered: In this review paper, we report the current status of the molecular diagnostics in the diagnosis of mucormycosis: serologic tests, IHC, PCR, protein-based with MALDI-TOF, metabolomics and metagenomic sequencing. Expert commentary: This review will conclude with an expert commentary on the potential uses/challenges of the currently available tests and the future of molecular diagnostics for mucormycosis.
引用
收藏
页码:845 / 854
页数:10
相关论文
共 74 条
[1]   Performance evaluation of multiplex PCR including Aspergillus - not so simple! [J].
Alanio, Alexandre ;
Bretagne, Stephane .
MEDICAL MYCOLOGY, 2017, 55 (01) :56-62
[2]   Prospective Evaluation of Serum β-Glucan Testing in Patients With Probable or Proven Fungal Diseases [J].
Angebault, Cecile ;
Lanternier, Fanny ;
Dalle, Frederic ;
Schrimpf, Cecile ;
Roupie, Anne-Laure ;
Dupuis, Aurelie ;
Scemla, Anne ;
Paubelle, Etienne ;
Caillot, Denis ;
Neven, Benedicte ;
Frange, Pierre ;
Suarez, Felipe ;
d'Enfert, Christophe ;
Lortholary, Olivier ;
Bougnoux, Marie-Elisabeth .
OPEN FORUM INFECTIOUS DISEASES, 2016, 3 (03)
[3]  
[Anonymous], COLD SPRING HARB PER
[4]  
[Anonymous], 2016, OPEN FORUM INFECT DI, DOI [DOI 10.1093/OFID/OFW209, 10.1093/ofid/ofw209]
[5]  
[Anonymous], MYCOSES
[6]  
[Anonymous], 28 ECCMID
[7]  
[Anonymous], OPEN FORUM INFECT DI
[8]  
[Anonymous], OPEN FORUM INFECT DI, DOI DOI 10.1093/OFID/OFW190.
[9]  
[Anonymous], 2015, CLIN MICROBIOL INFEC
[10]   Sequence-Based Identification of Aspergillus, Fusarium, and Mucorales Species in the Clinical Mycology Laboratory: Where Are We and Where Should We Go from Here? [J].
Balajee, S. A. ;
Borman, A. M. ;
Brandt, M. E. ;
Cano, J. ;
Cuenca-Estrella, M. ;
Dannaoui, E. ;
Guarro, J. ;
Haase, G. ;
Kibbler, C. C. ;
Meyer, W. ;
O'Donnell, K. ;
Petti, C. A. ;
Rodriguez-Tudela, J. L. ;
Sutton, D. ;
Velegraki, A. ;
Wickes, B. L. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (04) :877-884