Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system

被引：465

作者：

Ghorbal, Mehdi ^{[1
,2
]}

Gorman, Molly ^{[1
,2
]}

Macpherson, Cameron Ross ^{[1
,2
]}

Martins, Rafael Miyazawa ^{[1
,2
]}

Scherf, Artur ^{[1
,2
]}

Lopez-Rubio, Jose-Juan ^{[1
,2
]}

机构：

[1] Inst Pasteur, Biol Host Parasite Interact Unit, Paris, France

[2] Inst Pasteur, CNRS URA 2581, Paris, France

来源：

NATURE BIOTECHNOLOGY | 2014年 / 32卷 / 08期

基金：

欧洲研究理事会;

关键词：

GENE DELETION; ORC1; RNAS; DNA;

D O I：

10.1038/nbt.2925

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Genome manipulation in the malaria parasite Plasmodium falciparum remains largely intractable and improved genomic tools are needed to further understand pathogenesis and drug resistance. We demonstrated the CRISPR-Cas9 system for use in P. falciparum by disrupting chromosomal loci and generating marker-free, single-nucleotide substitutions with high efficiency. Additionally, an artemisinin-resistant strain was generated by introducing a previously implicated polymorphism, thus illustrating the value of efficient genome editing in malaria research.

引用

收藏

页码：819 / 821

页数：3

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