Overexpression of indoleamine 2, 3-dioxygenase contributes to the repair of human airway epithelial cells inhibited by dexamethasone via affecting the MAPK/ERK signaling pathway

被引：12

作者：

Jia, Shanshan ^{[1
]}

Guo, Pin ^{[2
]}

Ge, Xiangjin ^{[1
]}

Wu, Huanhuan ^{[3
]}

Lu, Junhua ^{[1
]}

Fan, Xiaofang ^{[4
]}

机构：

[1] Hengdian Wenrongs Hosp, Dept Respirat, 99 Guest Meeting Ave, Dongyang 322118, Zhejiang, Peoples R China

[2] Dong Yang Red Cross Hosp, Dept Urol, Jinhua 322100, Zhejiang, Peoples R China

[3] Dongyang Peoples Hosp, Dept Respirat, Jinhua 322100, Zhejiang, Peoples R China

[4] Wenzhou Med Univ, Cor Pulmonale Res Lab, Wenzhou 325035, Zhejiang, Peoples R China

来源：

EXPERIMENTAL AND THERAPEUTIC MEDICINE | 2018年 / 16卷 / 01期

关键词：

indoleamine; 2; 3-dioxygenase; asthma; 16HBE; airway epithelial cells; dexamethasone; MAPK; ERK; OBSTRUCTIVE PULMONARY-DISEASE; GLUCOCORTICOID-RECEPTOR; ANTIINFLAMMATORY ACTION; OVERLAP SYNDROME; DENDRITIC CELLS; 2,3-DIOXYGENASE; ASTHMA; EXPRESSION; CORTICOSTEROIDS; INFLAMMATION;

D O I：

10.3892/etm.2018.6163

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Indoleamine 2, 3-dioxygenase (IDO) catalyzes the degradation of trytophan, which serves a key role in immune suppression via regulating the production of several metabolites. The present study aimed to explore the effects and mechanisms of IDO in the repair of human airway epithelium suppressed by dexamethasone (DEX). Cell viability, proliferation and migration were evaluated using a Cell Counting Kit-8 (CCK-8), 5(6)-carboxyfluorescein diacetate succinimidyl ester (CFSE) labeling, and wound-healing assay, respectively. Reverse transcription-quantitative polymerase chain reaction (RT-VCR), western blot analysis and ELISA were performed to assess the levels of IDO, the mitogen-activated protein kinase (MAPK)/extracellular regulated kinase (ERK) pathway-related factors and epidermal growth factor (EGF) expression, respectively. The results revealed that overexpression of IDO enhanced the cell viability, and promoted the proliferation and migration of 16HBE cells which repair was inhibited by DEX. Furthermore, it was indicated that overexpression of IDO affected the MAPK/ERK pathway. In conclusion, overexpression of IDO promoted the human airway epithelium repair inhibited by DEX through affecting MAPK/ERK pathway. The present study implied that IDO may be a potential genetic therapeutic agent and supported the utilization of IDO in asthma.

引用

页码：282 / 290

页数：9

共 48 条

[21] The mitogen-activated/extracellular signal-regulated kinase kinase 1/2 inhibitor U0126 induces glial fibrillary acidic protein expression and reduces the proliferation and migration of C6 glioma cells [J].

Lind, C. R. P. ;

Gray, C. W. ;

Pearson, A. G. ;

Cameron, R. E. ;

O'Carroll, S. J. ;

Narayan, P. J. ;

Lim, J. ;

Dragunow, M. .

NEUROSCIENCE, 2006, 141 (04) :1925-1933

[22] Constitutive IDO expression in human cancer is sustained by an autocrine signaling loop involving IL-6, STAT3 and the AHR [J].

Litzenburger, Ulrike M. ;

Opitz, Christiane A. ;

Sahm, Felix ;

Rauschenbach, Katharina J. ;

Trump, Saskia ;

Winter, Marcus ;

Ott, Martina ;

Ochs, Katharina ;

Lutz, Christian ;

Liu, Xiangdong ;

Anastasov, Natasa ;

Lehmann, Irina ;

Hoefer, Thomas ;

von Deimling, Andreas ;

Wick, Wolfgang ;

Platten, Michael .

ONCOTARGET, 2014, 5 (04) :1038-1051

[23] Dexamethasone Inhibits Repair of Human Airway Epithelial Cells Mediated by Glucocorticoid-Induced Leucine Zipper (GILZ) [J].

Liu, Jingyue ;

Zhang, Mingxiang ;

Niu, Chao ;

Luo, Zhengxiu ;

Dai, Jihong ;

Wang, Lijia ;

Liu, Enmei ;

Fu, Zhou .

PLOS ONE, 2013, 8 (04)

[24] Indoleamine 2,3-Dioxygenase, an Emerging Target for Anti-Cancer Therapy [J].

Liu, X. ;

Newton, R. C. ;

Friedman, S. M. ;

Scherle, P. A. .

CURRENT CANCER DRUG TARGETS, 2009, 9 (08) :938-952

[25] Role of indoleamine 2,3-dioxygenase in antimicrobial defence and immuno-regulation:: Tryptophan depletion versus production of toxic kynurenines [J].

MacKenzie, C. R. ;

Heseler, K. ;

Mueller, A. ;

Daeubener, Walter .

CURRENT DRUG METABOLISM, 2007, 8 (03) :237-244

[26]

MILLER LA, 1993, CANCER RES, V53, P2527

[27] Activation of calcium-dependent kinases and epidermal growth factor receptor regulate muscarinic acetylcholine receptor-mediated MAPK/ERK activation in thyroid epithelial cells [J].

Montiel, Mercedes ;

Quesada, Juan ;

Jimenez, Eugenio .

CELLULAR SIGNALLING, 2007, 19 (10) :2138-2146

[28] Expression of indoleamine 2,3-dioxygenase by plasmacytoid dendritic cells in tumor-draining lymph nodes [J].

Munn, DH ;

Sharma, MD ;

Hou, D ;

Baban, B ;

Lee, JR ;

Antonia, SJ ;

Messina, JL ;

Chandler, P ;

Koni, PA ;

Mellor, AL .

JOURNAL OF CLINICAL INVESTIGATION, 2004, 114 (02) :280-290

[29] Network beyond IDO in psychiatric disorders: Revisiting neurodegeneration hypothesis [J].

Myint, Aye-Mu ;

Kim, Yong-Ku .

PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY, 2014, 48 :304-313

[30] Asthma, Chronic Obstructive Pulmonary Disease (COPD), and the Overlap Syndrome [J].

Nakawah, Mohammad Obadah ;

Hawkins, Clare ;

Barbandi, Farouk .

JOURNAL OF THE AMERICAN BOARD OF FAMILY MEDICINE, 2013, 26 (04) :470-477

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