3D-printed biological cell phantom for testing 3D quantitative phase imaging systems

被引：46
|
作者：
Ziemczonok, Michal [1 ]
Kus, Arkadiusz [1 ]
Wasylczyk, Piotr [2 ]
Kujawinska, Malgorzata [1 ]
机构：
[1] Warsaw Univ Technol, Inst Micromech & Photon, Sw A Boboli 8 St, PL-02525 Warsaw, Poland
[2] Univ Warsaw, Fac Phys, Photon Nanostruct Facil, Pasteura 5, PL-02093 Warsaw, Poland
来源：
SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期
关键词：
TOMOGRAPHIC DIFFRACTIVE MICROSCOPY; HOLOGRAPHIC TOMOGRAPHY; REFRACTIVE-INDEX; CONTRAST; RECONSTRUCTION; PRINCIPLES; RESOLUTION;
D O I：
10.1038/s41598-019-55330-4
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
As the 3D quantitative phase imaging (QPI) methods mature, their further development calls for reliable tools and methods to characterize and compare their metrological parameters. We use refractive index engineering during two-photon laser photolithography to fabricate a life-scale phantom of a biological cell with internal structures that mimic optical and structural properties of mammalian cells. After verification with a number of reference techniques, the phantom is used to characterize the performance of a limited-angle holographic tomography microscope.
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页数：9
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