Stroke Location and Brain Function in an Embolic Rabbit Stroke Model

被引:19
作者
Brown, Aliza T. [1 ]
Skinner, Robert D. [1 ,2 ]
Flores, Rene
Hennings, Leah [3 ]
Borrelli, Michael J. [1 ]
Lowery, John [4 ]
Culp, William C. [1 ]
机构
[1] Univ Arkansas Med Sci, Dept Radiol, Little Rock, AR 72205 USA
[2] Univ Arkansas Med Sci, Dept Neurobiol & Dev Sci, Little Rock, AR 72205 USA
[3] Univ Arkansas Med Sci, Dept Pathol, Little Rock, AR 72205 USA
[4] Univ Arkansas Med Sci, Dept Lab Anim Med, Little Rock, AR 72205 USA
基金
美国国家卫生研究院;
关键词
TISSUE-PLASMINOGEN-ACTIVATOR; CLINICAL RATING SCORES; MOTOR RECOVERY; CEREBRAL-HEMORRHAGE; ARTERY-OCCLUSION; ISCHEMIA; THROMBOEMBOLECTOMY; ANGIOGRAPHY; COMBINATION; SYNERGISM;
D O I
10.1016/j.jvir.2010.02.023
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
PURPOSE: Current rabbit stroke models often depend on symptoms as endpoints for embolization and produce wide variation in location, size, and severity of strokes. In a further refinement of an angiographic embolic stroke model, localized infarctions were correlated to neurologic deficits with the goal to create a rabbit model for long-term studies of therapies after stroke. MATERIALS AND METHODS: New Zealand White rabbits (4-5 kg; N = 71) had selective internal carotid artery (ICA) angiography and a single clot was injected. At 24 hours, neurologic assessment score (NAS) was measured on an 11-point scale (0, normal; 10, dead). Brains were removed and stained to identify stroke areas. All animals with single strokes (n = 31) were analyzed by specific brain structure involvement, and NAS values were correlated. RESULTS: Stroke incidence differed by location, with cortex, subcortical, and basal ganglia regions highest. The middle cerebral artery (MCA), at 52%, and anterior cerebral artery (ACA), at 29%, were most commonly involved, with the largest stroke volumes in the ACA distribution. Brainstem and cerebellum strokes had disproportionately severe neurologic deficits, scoring 2.25 +/- 1.0 on the NAS, which represented a significant (P < .02) difference versus cortex (0.5 +/- 0.2), subcortical (1.3 +/- 0.4), and basal ganglia (0.5 +/- 0.3), all in the frontal or parietal regions. CONCLUSIONS: MCA and ACA distributions included 81% of strokes. These sites were relatively silent (potentially allowing longer-term survival studies) whereas others in the posterior circulation produced disproportionately severe symptoms. Symptoms were not reliable indicators of stroke occurrence, and other endpoints such as imaging may be required. These are important steps toward refinement of the rabbit stroke model.
引用
收藏
页码:903 / 909
页数:7
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