The effectiveness of chemotherapy and electrochemotherapy on ovarian cell lines in vitro

被引：11

作者：

Saczko, J. ^{[1
]}

Pilat, J. ^{[1
]}

Choromanska, A. ^{[1
]}

Rembalkowska, N. ^{[1
]}

Bar, J. ^{[2
]}

Kaminska, I. ^{[2
]}

Zalewski, J. ^{[3
]}

Kulbacka, J. ^{[1
]}

机构：

[1] Wroclaw Med Univ, Dept Med Biochem, Wroclaw, Poland

[2] Wroclaw Med Univ, Dept Immunopathol & Mol Biol, Wroclaw, Poland

[3] Wroclaw Med Univ, Dept Gynecol & Obstet, Wroclaw, Poland

来源：

NEOPLASMA | 2016年 / 63卷 / 03期

关键词：

chemotherapy; electroporation; electrochemotherapy; bleomycin; ovarian cancer; NEGATIVE BREAST-CANCER; HEAT-SHOCK PROTEINS; CARCINOMA CELLS; COLORECTAL-CARCINOMA; GENE-THERAPY; ELECTROPORATION; BLEOMYCIN; EXPRESSION; CISPLATIN; DRUG;

D O I：

10.4149/315_150930N510

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The presented study aimed to evaluate in vitro the effectiveness of improvement standard chemotherapy with bleomycin by electroporation in two various ovarian cancer cell lines. Two human ovarian cell lines OvBH-1 and SKOV-3 were used. The lines were selected because of their resistance to several therapeutic methods. As anticancer drug we use range of concentrations of bleomycin. In EP and ECT experiments different voltage values: from 0 to 1200 V/cm, 8 pulses with duration of 100 mu s and intervals between pulses is long were used. The cells viability after applied treatments was evaluated by MTT assay. The expression of heat shock proteins - HSP27 was examined by immunocytochemical ABC method. The cytotoxicity with different concentrations of bleomycin alone was not significantly decrease in both cell lines. It confirms resistance of these cells to conventional chemotherapy. The highest decrease of cell proliferation was observed after EP with bleomycin after 48h of incubation for 1000 V/cm. The intensity of expression of small heat shock proteins HSP27 slightly increased after ECT in both treated cell lines, in particular in OvBH-1. The presented study indicated that application of electroporation may effectively enhance chemotherapy with bleomycin, particularly in the case of treating ovarian cancer resistant to standard therapy.

引用

页码：450 / 455

页数：6

共 40 条

[1] Protective role of Hsp27 protein against γ radiation-induced apoptosis and radiosensitization effects of Hsp27 gene silencing in different human tumor cells [J].