A complex virome unveiled by deep sequencing analysis of RNAs from a French Pinot Noir grapevine exhibiting strong leafroll symptoms

被引：29

作者：

Beuve, Monique ^{[1
]}

Hily, Jean-Michel ^{[1
]}

Alliaume, Antoine ^{[1
]}

Reinbold, Catherine ^{[1
]}

Le Maguet, Jean ^{[1
,2
]}

Candresse, Thierry ^{[3
]}

Herrbach, Etienne ^{[1
]}

Lemaire, Olivier ^{[1
]}

机构：

[1] Univ Strasbourg, SVQV, F-68000 Colmar, France

[2] IFPC, F-61500 Sees, France

[3] Univ Bordeaux, INRA, UMR Biol Fruit & Pathol 1332, Villenave Dornon, France

来源：

ARCHIVES OF VIROLOGY | 2018年 / 163卷 / 11期

关键词：

PITTING-ASSOCIATED-VIRUS; NUCLEOTIDE-SEQUENCE; PHENACOCCUS-ACERIS; GENETIC DIVERSITY; IDENTIFICATION; VITIVIRUS; VARIANTS; GENOME; VINEYARDS; DISEASE;

D O I：

10.1007/s00705-018-3949-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have characterized the virome of a grapevine Pinot Noir accession (P70) that displayed, over the year, very stable and strong leafroll symptoms. For this, we have used two extraction methods (dsRNA and total RNA) coupled with the high throughput sequencing (HTS) Illumina technique. While a great disparity in viral sequences were observed, both approaches gave similar results, revealing a very complex infection status. Five virus and viroid isolates [Grapevine leafroll-associated viruse-1 (GLRaV-1), Grapevine virus A (GVA), Grapevine rupestris stem pitting-associated virus (GRSPaV), Hop stunt viroid (HSVd) and Grapevine yellow speckle viroid 1 (GYSVd1)] were detected in P70 with a grand total of eleven variants being identified and de novo assembled. A comparison between both extraction methods regarding their power to detect viruses and the ease of genome assembly is also provided.

引用

页码：2937 / 2946

页数：10

共 52 条

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