Berberine protects homocysteic acid-induced HT-22 cell death: involvement of Akt pathway

被引：18

作者：

Chen, Meihui ^{[1
]}

Tan, Min ^{[1
,2
]}

Jing, Minghua ^{[3
]}

Liu, Anmin ^{[4
]}

Liu, Qinyu ^{[4
]}

Wen, Shijun ^{[1
,5
]}

Chen, Ziwei ^{[1
]}

Chao, Xiaojuan ^{[1
]}

He, Xixin ^{[2
]}

Ramassamy, Charles ^{[6
]}

Gao, Youheng ^{[2
]}

Pi, Rongbiao ^{[1
]}

机构：

[1] Sun Yat Sen Univ, Sch Pharmaceut Sci, Dept Pharmacol & Toxicol, Guangzhou 510006, Guangdong, Peoples R China

[2] Guangzhou Univ Chinese Med, Dept Tradit Chinese Med Chem, Coll Chinese Mat Madica, Guangzhou 510006, Guangdong, Peoples R China

[3] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Tradit Chinese Med, Guangzhou 510006, Guangdong, Peoples R China

[4] Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Guangzhou 510275, Guangdong, Peoples R China

[5] Sun Yat Sen Univ Canc Ctr, State Key Lab Oncol South China, Guangzhou 510060, Guangdong, Peoples R China

[6] INRS Inst Armand Frappier, Laval, PQ, Canada

来源：

METABOLIC BRAIN DISEASE | 2015年 / 30卷 / 01期

关键词：

Berberine; Alzheimer's disease; Homocysteic acid; Cytotoxicity; Akt; ALZHEIMERS-DISEASE; INDUCED CYTOTOXICITY; SIGNALING PATHWAY; ACTIVATION; NEURODEGENERATION; MECHANISMS; SURVIVAL; DAMAGE; MODEL; MICE;

D O I：

10.1007/s11011-014-9580-x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Berberine (BBR), one of the major constituents of Chinese herb Rhizoma coptidis, has been reported to exert beneficial effects to various diseases, including Alzheimer's disease (AD). In the present work, we aimed to investigate the effects of BBR on neuronal cell death induced by homocysteic acid (HCA), which was considered as a risk of AD. BBR significantly reduced HCA-induced reactive oxygen species (ROS) generation, lactate dehydrogenase release and subsequent cell death. LY294002, the PI3K inhibitor, blocked the protection as well as the up-regulation of Akt phosphorylation of BBR. Taken together, our results indicate that BBR protects HCA-induced HT-22 cell death partly via modulating Akt pathway, suggesting BBR may be a promising therapeutic agent for the treatment of HCA-related diseases, including AD.

引用

页码：137 / 142

页数：6

共 20 条

[1] Berberine protects 6-hydroxydopamine-induced human dopaminergic neuronal cell death through the induction of heme oxygenase-1 [J].

Bae, Jinbum ;

Lee, Danbi ;

Kim, Yun Kyu ;

Gil, Minchan ;

Lee, Joo-Yong ;

Lee, Kyung Jin .

MOLECULES AND CELLS, 2013, 35 (02) :151-157

[2] Transcription-dependent and -independent control of neuronal survival by the PI3K-Akt signaling pathway [J].

Brunet, A ;

Datta, SR ;

Greenberg, ME .

CURRENT OPINION IN NEUROBIOLOGY, 2001, 11 (03) :297-305

[3] PROTEIN-KINASE-C ACTIVATION INHIBITS GLUTAMATE-INDUCED CYTOTOXICITY IN A NEURONAL CELL-LINE [J].

DAVIS, JB ;

MAHER, P .

BRAIN RESEARCH, 1994, 652 (01) :169-173

[4] Mitochondrial complex I inhibition in cerebral cortex of immature rats following homocysteic acid-induced seizures [J].

Folbergrova, Jaroslava ;

Jesina, Pavel ;

Drahota, Zdenek ;

Lisy, Vaclav ;

Haugvicova, Renata ;

Vojtiskova, Alena ;

Houstek, Josef .

EXPERIMENTAL NEUROLOGY, 2007, 204 (02) :597-609

[5] Homocysteic acid induces intraneuronal accumulation of neurotoxic Aβ42:: Implications for the pathogenesis of Alzheimer's disease [J].

Hasegawa, T ;

Ukai, W ;

Jo, DG ;

Xu, XR ;

Mattson, MP ;

Nakagawa, M ;

Araki, W ;

Saito, T ;

Yamada, T .

JOURNAL OF NEUROSCIENCE RESEARCH, 2005, 80 (06) :869-876

[6] Treatment of Alzheimer's Disease with Anti-Homocysteic Acid Antibody in 3xTg-AD Male Mice [J].

Hasegawa, Tohru ;

Mikoda, Nobuyuki ;

Kitazawa, Masashi ;

LaFerla, Frank M. .

PLOS ONE, 2010, 5 (01)

[7] Berberine activates Nrf2 nuclear translocation and protects against oxidative damage via a phosphatidylinositol 3-kinase/Akt-dependent mechanism in NSC34 motor neuron-like cells [J].

Hsu, Ya-Yun ;

Chen, Cheng-Sheng ;

Wu, Sheng-Nan ;

Jong, Yuh-Jyh ;

Lo, Yi-Ching .

EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2012, 46 (05) :415-425

[8] Motor neuron degeneration induced by excitotoxin agonists has features in common with those seen in the SOD-1 transgenic mouse model of amyotrophic lateral sclerosis [J].

Ikonomidou, C ;

Qin, YQ ;

Labruyere, J ;

Olney, JW .

JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY, 1996, 55 (02) :211-224

[9] Isoquercitrin is the most effective antioxidant in the plant Thuja orientalis and able to counteract oxidative-induced damage to a transformed cell line (RGC-5 cells) [J].

Jung, Sang Hoon ;

Kim, Beum Jin ;

Lee, Eun Ha ;

Osborne, Neville N. .

NEUROCHEMISTRY INTERNATIONAL, 2010, 57 (07) :713-721

[10] Berberine: A Plant Alkaloid with Therapeutic Potential for Central Nervous System Disorders [J].

Kulkarni, S. K. ;

Dhir, Ashish .

PHYTOTHERAPY RESEARCH, 2010, 24 (03) :317-324

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