Determination of glucosamine in nutritional supplements by reversed-phase ion-pairing HPLC

被引：20

作者：

Way, WK ^{[1
]}

Gibson, KG ^{[1
]}

Breite, AG ^{[1
]}

机构：

[1] Chemir Polytech Labs Inc, Maryland Hts, MO 63043 USA

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2000年 / 23卷 / 18期

关键词：

D O I：

10.1081/JLC-100101238

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reverse-phase high performance liquid chromatography (HPLC) method for the determination of glucosamine in nutritional supplements was developed and validated based on USP guidelines. Separations were performed using a MetaChem Inertsil ODS-3 column with an isocratic mobile phase consisting of 10% Methanol, 90% 0.005M octanesulfonate, pH = 2.1. Glucosamine was detected using a refractive index (RI) detector. The octanesulfonate ion pairing agent allowed for retention on a C-18 column. The method proved highly reliable with respect to standard performance characteristics. The method allows for convenient quality control in the growing nutritional supplement market.

引用

页码：2861 / 2871

页数：11

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