DNA-polyfluorophore excimers as sensitive reporters for esterases and lipases

被引：29
作者：
Dai, Nan [1 ]
Teo, Yin Nah [1 ]
Kool, Eric T. [1 ]
机构：
[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA
来源：
CHEMICAL COMMUNICATIONS | 2010年 / 46卷 / 08期
基金：
美国国家卫生研究院;
关键词：
INTRAMOLECULAR DIMERS; SEQUENCE DEPENDENCE; FLUORESCENCE; LABELS; INHIBITORS; BACKBONE; PRODRUGS; ENZYMES; PROBES;
D O I：
10.1039/b926338a
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
DNA-scaffolded oligodeoxyriboside fluorophores (ODFs) were used as the reporters in turn-on sensing of enzymatic bond-cleaving activity. A tetramer ODF of pyrene deoxynucleosides displayed high quenching efficiency when conjugated via ester linkages with a dabcyl quencher, and yielded large signal increases with several enzymes in vitro and in intact human cells.
引用
收藏
页码：1221 / 1223
页数：3
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