Ploidy-dependent changes in the epigenome of symbiotic cells correlate with specific patterns of gene expression

被引：44

作者：

Nagymihaly, Marianna ^{[1
,2
]}

Veluchamy, Alaguraj ^{[3
]}

Gyoergypal, Zoltan ^{[4
]}

Ariel, Federico ^{[5
]}

Jegu, Teddy ^{[6
]}

Benhamed, Moussa ^{[6
]}

Szucs, Attila ^{[1
]}

Kereszt, Attila ^{[1
]}

Mergaert, Peter ^{[2
]}

Kondorosi, Eva ^{[1
,2
]}

机构：

[1] Hungarian Acad Sci, Biol Res Ctr, Inst Biochem, H-6726 Szeged, Hungary

[2] Univ Paris Sud, CNRS, Inst Integrat Biol Cell,UMR9198, Commissariat Energie Atom & Energies Alternat, F-91198 Gif Sur Yvette, France

[3] King Abdullah Univ Sci & Technol, Div Biol & Environm Sci & Engn, Thuwal 239556900, Saudi Arabia

[4] Hungarian Acad Sci, Biol Res Ctr, Inst Biophys, H-6726 Szeged, Hungary

[5] Univ Nacl Litoral, Fac Bioquim & Ciencias Biol, Inst Agrobiotecnol Litoral, Consejo Nacl Invest Cient & Tecn, RA-3000 Santa Fe, Argentina

[6] Univ Paris Saclay, Univ Paris Diderot, Sorbonne Paris Cite,Inst Plant Sci Paris Saclay, Univ Evry,Univ Paris Sud,CNRS,French Natl Inst Ag, F-91405 Orsay, France

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2017年 / 114卷 / 17期

基金：

欧洲研究理事会;

关键词：

Medicago truncatula; symbiosis; nodule-specific cysteine-rich peptides; DNA methylation; chromatin structure; REVEALS DNA METHYLATION; GENOME-WIDE ANALYSIS; MEDICAGO-TRUNCATULA; ROOT-NODULES; ARABIDOPSIS; DIFFERENTIATION; CHROMATIN; PLANT; ENDOREDUPLICATION; TRANSCRIPTION;

D O I：

10.1073/pnas.1704211114

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The formation of symbiotic nodule cells in Medicago truncatula is driven by successive endoreduplication cycles and transcriptional reprogramming in different temporal waves including the activation of more than 600 cysteine-rich NCR genes expressed only in nodules. We show here that the transcriptional waves correlate with growing ploidy levels and have investigated how the epigenome changes during endoreduplication cycles. Differential DNA methylation was found in only a small subset of symbiotic nodule-specific genes, including more than half of the NCR genes, whereas in most genes DNA methylation was unaffected by the ploidy levels and was independent of the genes' active or repressed state. On the other hand, expression of nodule-specific genes correlated with ploidy-dependent opening of the chromatin as well as, in a subset of tested genes, with reduced H3K27me3 levels combined with enhanced H3K9ac levels. Our results suggest that endoreduplication-dependent epigenetic changes contribute to transcriptional reprogramming in the differentiation of symbiotic cells.

引用

页码：4543 / 4548

页数：6

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