Post-analysis follow-up and validation of microarray experiments

被引：305
作者：
Chuaqui, RF
Bonner, RF
Best, CJM
Gillespie, JW
Flaig, MJ
Hewitt, SM
Phillips, JL
Krizman, DB
Tangrea, MA
Ahram, M
Linehan, WM
Knezevic, V
Emmert-Buck, MR [1 ]
机构：
[1] NCI, Pathogenet Unit, Pathol Lab, Bethesda, MD 20892 USA
[2] NCI, Urol Oncol Branch, Ctr Canc Res, Bethesda, MD 20892 USA
来源：
NATURE GENETICS | 2002年 / 32卷 / Suppl 4期
关键词：
D O I：
10.1038/ng1034
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Measurement of gene-expression profiles using microarray technology is becoming increasingly popular among the biomedical research community. Although there has been great progress in this field, investigators are still confronted with a difficult question after completing their experiments: how to validate the large data sets that are generated? This review summarizes current approaches to verifying global expression results, discusses the caveats that must be considered, and describes some methods that are being developed to address outstanding problems.
引用
收藏
页码：509 / 514
页数：6
相关论文
共 73 条
[21]  
2-2
[22]   Molecular profiling of clinical tissue specimens -: Feasibility and applications [J].
Emmert-Buck, MR ;
Strausberg, RL ;
Krizman, DB ;
Bonaldo, MF ;
Bonner, RF ;
Bostwick, DG ;
Brown, MR ;
Buetow, KH ;
Chuaqui, RF ;
Cole, DA ;
Duray, PH ;
Englert, CR ;
Gillespie, JW ;
Greenhut, S ;
Grouse, L ;
Hillier, LW ;
Katz, KS ;
Klausner, RD ;
Kuznetzov, V ;
Lash, AE ;
Lennon, G ;
Linehan, WM ;
Liotta, LA ;
Marra, MA ;
Munson, PJ ;
Ornstein, DK ;
Prabhu, VV ;
Prange, C ;
Schuler, GD ;
Soares, MB ;
Tolstoshev, CM ;
Vocke, CD ;
Waterston, RH .
AMERICAN JOURNAL OF PATHOLOGY, 2000, 156 (04) :1109-1115
[23]   Laser capture microdissection [J].
EmmertBuck, MR ;
Bonner, RF ;
Smith, PD ;
Chuaqui, RF ;
Zhuang, ZP ;
Goldstein, SR ;
Weiss, RA ;
Liotta, LA .
SCIENCE, 1996, 274 (5289) :998-1001
[24]  
Englert CR, 2000, CANCER RES, V60, P1526
[25]   Frozen tumor tissue microarray technology for analysis of tumor RNA, DNA, and proteins [J].
Fejzo, MS ;
Slamon, DJ .
AMERICAN JOURNAL OF PATHOLOGY, 2001, 159 (05) :1645-1650
[26]   Immuno-LCM: Laser capture microdissection of immunostained frozen sections for mRNA analysis [J].
Fend, F ;
Emmert-Buck, MR ;
Chuaqui, R ;
Cole, K ;
Lee, J ;
Liotta, LA ;
Raffeld, M .
AMERICAN JOURNAL OF PATHOLOGY, 1999, 154 (01) :61-66
[27]   Microarrays: handling the deluge of data and extracting reliable information [J].
Hess, KR ;
Zhang, W ;
Baggerly, KA ;
Stivers, DN ;
Coombes, KR .
TRENDS IN BIOTECHNOLOGY, 2001, 19 (11) :463-468
[28]   IL-2 and IL-12 alter NK cell responsiveness to IFN-γ-inducible protein 10 by down-regulating CXCR3 expression [J].
Hodge, DL ;
Schill, WB ;
Wang, JM ;
Blanca, I ;
Reynolds, DA ;
Ortaldo, JR ;
Young, HA .
JOURNAL OF IMMUNOLOGY, 2002, 168 (12) :6090-6098
[29]   Options available - from start to finish - for obtaining data from DNA microarrays [J].
Holloway, AJ ;
van Laar, RK ;
Tothill, RW ;
Bowtell, DDL .
NATURE GENETICS, 2002, 32 (Suppl 4) :481-489
[30]   Tissue microarray profiling of cancer specimens and cell lines: Opportunities and limitations [J].
Hoos, A ;
Cordon-Cardo, C .
LABORATORY INVESTIGATION, 2001, 81 (10) :1331-1338
12345678