Skin tolerant inactivation of multiresistant pathogens using far-UVC LEDs

被引：73

作者：

Glaab, Johannes ^{[1
]}

Lobo-Ploch, Neysha ^{[1
]}

Cho, Hyun Kyong ^{[1
]}

Filler, Thomas ^{[1
]}

Gundlach, Heiko ^{[3
]}

Guttmann, Martin ^{[2
]}

Hagedorn, Sylvia ^{[1
]}

Lohan, Silke B. ^{[4
,5
,6
]}

Mehnke, Frank ^{[2
,8
]}

Schleusener, Johannes ^{[4
,5
,6
]}

Sicher, Claudia ^{[7
]}

Sulmoni, Luca ^{[2
]}

Wernicke, Tim ^{[2
]}

Wittenbecher, Lucas ^{[1
]}

Woggon, Ulrike ^{[3
]}

Zwicker, Paula ^{[7
]}

Kramer, Axel ^{[7
]}

Meinke, Martina C. ^{[4
,5
,6
]}

Kneissl, Michael ^{[1
,2
]}

Weyers, Markus ^{[1
]}

Winterwerber, Ulrike ^{[1
]}

Einfeldt, Sven ^{[1
]}

机构：

[1] Ferdinand Braun Inst gGmbH, Leibniz Inst Hochstfrequenztech, Gustav Kirchhoff Str 4, D-12489 Berlin, Germany

[2] Tech Univ Berlin, Inst Festkorperphys, Hardenbergstr 36, D-10623 Berlin, Germany

[3] Tech Univ Berlin, Inst Opt & Atomare Phys, Str 17,Juni 135, D-10623 Berlin, Germany

[4] Charite Univ Med Berlin, Dept Dermatol Venerol & Allergol, Ctr Expt & Appl Cutaneous Physiol, Charitepl 1, D-10117 Berlin, Germany

[5] Free Univ Berlin, Charitepl 1, D-10117 Berlin, Germany

[6] Humboldt Univ, Charitepl 1, D-10117 Berlin, Germany

[7] Univ Med Greifswald, Inst Hyg & Umweltmed, Ferdin & Sauerbruch Str, D-17475 Greifswald, Germany

[8] Georgia Inst Technol, Atlanta, GA 30332 USA

来源：

SCIENTIFIC REPORTS | 2021年 / 11卷 / 01期

关键词：

ULTRAVIOLET-RADIATION; BACTERIA; IRRADIATION; VIRUSES; ALN;

D O I：

10.1038/s41598-021-94070-2

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Multiresistant pathogens such as methicillin-resistant Staphylococcus aureus (MRSA) cause serious postoperative infections. A skin tolerant far-UVC (< 240 nm) irradiation system for their inactivation is presented here. It uses UVC LEDs in combination with a spectral filter and provides a peak wavelength of 233 nm, with a full width at half maximum of 12 nm, and an irradiance of 44 mu W/cm(2). MRSA bacteria in different concentrations on blood agar plates were inactivated with irradiation doses in the range of 15-40 mJ/cm(2). Porcine skin irradiated with a dose of 40 mJ/cm(2) at 233 nm showed only 3.7% CPD and 2.3% 6-4PP DNA damage. Corresponding irradiation at 254 nm caused 15-30 times higher damage. Thus, the skin damage caused by the disinfectant doses is so small that it can be expected to be compensated by the skin's natural repair mechanisms. LED-based far-UVC lamps could therefore soon be used in everyday clinical practice to eradicate multiresistant pathogens directly on humans.

引用

页数：11

共 53 条

[1] Decontamination of Targeted Pathogens from Patient Rooms Using an Automated Ultraviolet-C-Emitting Device [J].