The Chemokine CXCL16 Is a New Biomarker for Lymph Node Analysis of Colon Cancer Outcome

被引：24

作者：

AbdelMageed, Manar ^{[1
,2
,3
]}

Ali, Haytham ^{[1
,2
,3
]}

Olsson, Lina ^{[1
]}

Lindmark, Gudrun ^{[4
]}

Hammarstroem, Marie-Louise ^{[1
]}

Hammarstrom, Sten ^{[1
]}

Sitohy, Basel ^{[1
,2
]}

机构：

[1] Umea Univ, Dept Clin Microbiol Infect & Immunol, SE-90185 Umea, Sweden

[2] Umea Univ, Dept Radiat Sci, Oncol, SE-90185 Umea, Sweden

[3] Zagazig Univ, Dept Pathol, Fac Vet Med, Zagazig 44511, Egypt

[4] Lund Univ, Inst Clin Sci, SE-25187 Helsingborg, Sweden

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2019年 / 20卷 / 22期

基金：

瑞典研究理事会;

关键词：

chemokines; CXCL17; CEA; EpCAM; qRT-PCR; immunohistochemistry; immunomorphometry; CELL SUBSETS; EXPRESSION; GENES; RNA;

D O I：

10.3390/ijms20225793

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chemokines are important in the development and progression of tumors. We investigated the expression of CXCL14 and CXCL16 in colon cancer. Expression of mRNA was assessed in primary tumors and lymph nodes and CXCL16 mRNA levels were correlated to patient's survival. Protein expression was investigated by two-color immunofluorescence and immunomorphometry. CXCL14 and CXCL16 mRNA levels and protein expression were significantly higher in colon cancer primary tumors compared to apparently normal colon tissue. Positive cells were tumor cells, as revealed by anti-CEA and anti-EpCAM staining. CXCL16, but not CXCL14, mRNA levels were significantly higher in hematoxylin and eosin positive (H&E(+)) compared to H&E(-) colon cancer lymph nodes or control nodes (P < 0.0001). CXCL16 mRNA was expressed in 5/5 colon cancer cell lines while CXCL14 was expressed significantly in only one. Kaplan-Meier analysis revealed that colon cancer patients with lymph nodes expressing high or very high levels (7.2 and 11.4 copies/18S rRNA unit, respectively) of CXCL16 mRNA had a decreased mean survival time of 30 and 46 months at the 12-year follow-up (P = 0.04, P = 0.005, respectively). In conclusion, high expression of CXCL16 mRNA in regional lymph nodes of colon cancer patients is a sign of a poor prognosis.

引用

页数：16

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