Freedom of expression: cell-type-specific gene profiling

被引：7
|
作者：
Otsuki, Leo
Cheetham, Seth W.
Brand, Andrea H. [1 ]
机构：
[1] Univ Cambridge, Gurdon Inst, Cambridge, England
来源：
WILEY INTERDISCIPLINARY REVIEWS-DEVELOPMENTAL BIOLOGY | 2014年 / 3卷 / 06期
基金：
英国惠康基金;
关键词：
NEURAL STEM-CELLS; MESSENGER-RNA; FACS PURIFICATION; MIRNA INTERACTOME; MAMMALIAN-CELLS; COMPLEX TISSUES; SORTING MACS; IN-VIVO; DROSOPHILA; REVEALS;
D O I：
10.1002/wdev.149
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Cell fate and behavior are results of differential gene regulation, making techniques to profile gene expression in specific cell types highly desirable. Many methods now enable investigation at the DNA, RNA and protein level. This review introduces the most recent and popular techniques, and discusses key issues influencing the choice between these such as ease, cost and applicability of information gained. Interdisciplinary collaborations will no doubt contribute further advances, including not just in single cell type but single-cell expression profiling. (C) 2014 Wiley Periodicals, Inc.
引用
收藏
页码：429 / 443
页数：15
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