Molecular characterization and distribution of CYCLE protein from Athalia rosae

被引：8

作者：

Bembenek, Jadwiga ^{[1
]}

Itokawa, Kentaro ^{[1
]}

Hiragaki, Susumu ^{[1
]}

Shao, Qi-Miao ^{[1
]}

Tufail, Muhammad ^{[1
]}

Takeda, Makio ^{[1
]}

机构：

[1] Kobe Univ, Grad Sch Sci & Technol, Nada Ku, Kobe, Hyogo 6578501, Japan

来源：

JOURNAL OF INSECT PHYSIOLOGY | 2007年 / 53卷 / 05期

关键词：

sawfly; Atholia rosae; cycle; CYCLE (CYC); circadian clock; DROSOPHILA CLOCK GENE; FEEDBACK LOOP; MOUSE CLOCK; TRANSCRIPTION; HYMENOPTERA; SEQUENCE; PERIOD; PAS; ENTRAINMENT; EXPRESSION;

D O I：

10.1016/j.jinsphys.2006.12.013

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

cDNA encoding CYCLE (CYC) from the coleseed sawfly, Athalia rosae (Hymenoptera, Symphyta), was amplified by PCR. This is a first determination of hymenopteran CYC structure. ArCYC had an overall identity of 66% with CYC of Anopheles gambiae and ca. 60% of Drosophila melanoyaster. Structural investigation revealed that ArCYC contained characteristic motifs of: bHLH, PAS A, PAS B, PAC and BCTR. Detailed analysis indicated high conservation of these regions among insects. Northern blot analysis showed that the mRNA of ca. 3 kb was transcribed both in the head and in the rest of the body. Southern blot analysis suggested the presence of a single copy of the gene in the genome. Western blot indicates that the quantity of CYC protein does not fluctuate under LD 12:12 in either the head or the rest of the body. Immunocytochemical examination revealed CYC-like antigen in the pars intercerebralis, dorsolateral protocerebrum, dorsal optic tract, tritocerebrum of the brain and the subesophageal ganglion. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：418 / 427

页数：10

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